Garlic Powder Supplementation: Effects on Nutrient Utilization and Haematological Parameters of Sutchi catfish (Pangasianodon hypophthalmus, Sauvage, 1878)


62 / 8

Authors

  • P.P. Patel Kamdhenu University, Gujarat
  • R.V.Borichangar Kamdhenu University, Gujarat
  • V. R. Patel Kamdhenu University, Gujarat
  • S. C.Timbadia Kamdhenu University, Gujarat

Keywords:

catfish, Garlic, digestibility, nutrient utilization, Haematological parameters

Abstract

The experiment was conducted to evaluate the effect of garlic supplementation on nutrients utilization and haematological assay on Sutchi catfish (Pangasianodon hypophthalmus, sauvage, 1878). In first part of experiment total 500 number of P. hypophthalmus fingerlings with mean body weight (BW) of 27.74 ±1.46g and 134 ±1.41 mm of length (l) were distributed in four dietary treatment group with five replica in each as T1, T2, T3 and T4, respectively for a period of 60 days to access the nutrient utilization. In second part of the study, the same number at the P. hypophthalmus juveniles with 202.67 ±10.32 g BW and 270 ±4.27 mm were reared for 45 days to evaluate haematological parameters. In both the parts of the study the experimental fishes were supplemented with garlic (Allium sativum) powder (GP) in diet at the rate of 0, 1, 2 and 3%, respectively and reared in FRP tanks of 100 lit capacities for respective periods. Results revealed that significantly higher (P<0.05) feed assimilation efficiency (FAE), apparent digestibility co-efficient (%, ADC), nitrogen utilization (%) and digestible energy (kcal/g) was observed in T3 group supplemented with 2% GP. Significant changes in major blood biochemical and haematological parameters were observed on 45th day of experiment, where higher (P>0.05) values were observed for red blood cells (RBC), thrombocytes, packed cell volume (PCV), and haemoglobin (Hb) and lower (P<0.05) at 2% GP supplementation, however PCV and mean corpuscular value (MCV) were found higher at 3% GP supplementation (T4 group). No significant effect (P>0.05) of GP supplementation was revealed for monocyte, mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). It was concluded that 2% GP supplementation improves the feed utilization and health of Sutchi catfish.

Author Biographies

  • R.V.Borichangar, Kamdhenu University, Gujarat

    Principal, Fisheries College, Kamdhenu University, Navsari campus, Navsari

  • V. R. Patel , Kamdhenu University, Gujarat

    Assistant Professor, Department of Animal Nutrition

References

Adler, A.J., and Holub, B.J. 1997. Effect of garlic and fish-oil supplementation on serum lipid and lipoprotein concentrations in hypercholesterolemicmen. Am. J. Clin. Nutr. 65(2): 445-450.

A. O. A. C. 2005. Official Methods of Analysis of Association of Official Analytical Chemists, 18th edition, Maryland, USA.

Ahmed, N. and Hasan, M.R.2007.Growing pangas industry faces constraints in Bangladesh. Glob. Aquacult. Advocate.10: 60-62.

Ali, M.L., Haque, S.M., Wahab, M.A. and Borski, R.J. 2015. Development of Pangasius catfish culture technology in hyposaline waters. Aquanews.30(3): 1-4.

Ali, M. and Ibrahim, I. S. 2019. Phytochemical screening and proximate analysis of garlic (Allium sativum). Inorg Chem. 4: 478-482.

Aly, S. M., & Mohamed, M. F. (2010). Echinacea purpurea & Allium sativum as immunostimulants in fish culture using Nile tilapia (Oreochromis niloticus). Journal of Animal Physiology and Animal Nutrition, 94(5): 31-39

Biswas, C., Hossain, M.M., Jaman, H.U., Roy, H.S., Alam, F., Bappa, S.B. and Khatun, A. 2016. Dietary probiotics enhance the immunity of Thai pangas (Pangasiushypophthalmus) against Pseudomonas fluorescens.Int. j. fish. aquat. Sci. 4(4): 173-178.

Blaxhall, P.C. andDaisley, K.W. 1973. Routine haematological methods for use with fish blood. J. Fish Biol. 5(6): 771-781.

Cullen, S.P., Monahan, F.J., Callan, J.J. and O’Doherty, J.V. 2005. The effect of dietary garlic and rosemary on grower-finisher pig performance and sensory characteristics of pork. Irish J. Agric. Food Res. 44: 57-67.

Dabrowski, K. 1986. Physiological and nutritional aspects. Aquac. cyprinids, 55.

Da, C.T., Hung, L.T., Berg, H., Lindberg, J.E. and Lundh, T. 2013. Evaluation of potential feed sources, and technical and economic considerations of small‐scale commercial striped catfish (Pangasius hypothalamus) pond farming systems in the Mekong Delta of Vietnam. Aquac. Res. 44(3): 427-438.

Dacie, J.V. and Lewis, S.N.1984. Practical Haematology. 6th Edition. Edinburg, Churchill Livingstone.

Darwish, A.M., Mitchell, A.J. and Straus, D.L.2009. Evaluation of potassium permanganate against an experimental subacute infection of Flavobacteriumcolumnare in channel catfish, Ictaluruspunctatus (Rafinesque). J. Fish Dis. 32(2): 193-199.

De la Noue, J. andChoubert, G. 1986. Digestibility in rainbow trout: comparison of the direct and indirect methods of measurement. TheProg. Fish-Cult.48(3): 190-195.

Erguig M, Yahyaoui A, Fekhaoui M, Dakki M. 2015. The use of garlic in aquaculture. European j. biotechnol. biosci.3(8):28-33.

Esmaeili, M., AbedianKenari, A. and Rombenso, A.N.2017. Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchusmykiss,Walbaum, 1792). Aquac. Nutr.23(6), 1225-1234.

FAO. 2020.Cultured Aquatic Species Information Programme. Pangasiushypophthalmus. Text by D. Griffiths, P. Van Khanh and T.Q. Trong. In FAO Fisheries and Aquaculture Department [online]. Rome. (Accessed 23 March 2020). Available: http://www.fao.org/fishery/culturedspecies/Pangasius_hypophthalmus/en.

Fletcher, R. 2020, October 11. Catfish can be key drivers of global aquaculture growth. The fish site blog. https://thefishsite.com/articles/catfish-can-be-key-drivers-of-global-aquaculture-growth

Galagarza, O.A., Kuhn, D.D., Smith, S.A. and Hrubec, T.C.2017. Hematologic and plasma chemistry RIs for cultured striped catfish (Pangasiushypophthalmus) in recirculating aquaculture systems. Vet. Clin. Pathol. 46(3): 457-465.

Gilannejad, N., Silva, T., Rodriguez, G.M. and Yufera, M. 2019. Effect of feeding time and frequency on gut transit and feed digestibility in two fish species with different feeding behaviours, gilthead seabream and Senegalese sole. Aquac.513: 734438

Hien, T., Nguyen, P.T., Le Tu, T. and Glencross, B. 2010. Assessment of methods for the determination of digestibility of feed ingredients for Tra catfish, Pangasinodon hypothalamus. Aquac. Nutr.16: 351-358.

Huang, W., Yao, C., Liu, Y., Xu, N., Yin, Z., Xu, W., Miao, Y. Mal, K. and Ai, Q. 2020. Dietary allicin improved the survival and growth of large yellow croaker (Larimichthyscrocea) larvae via promoting intestinal development, alleviating inflammation and enhancing appetite. Front. Physiol.11:587674.

Hung, L. T., Liem, P. T., Tu, H. T. and Mariojouls, C.2002. Comparing growth and protein requirements for fingerlings of three catfish of the Mekong River (Pangasiusbocourti, Pangagasiushypothalmus and Pangasiusconchophilus). J.Aquacult.Trop. 17(4): 325-335.

Jahanjoo, V., Yahyavi, M., Akrami, R. and Bahri, A. H. 2018. Influence of adding garlic (Allium sativum), Ginger (Zingiberofficinale), thyme (Thymus vulgaris) and their combination on the growth performance, haematoimmunologicalparameters and disease resistance to Photobacteriumdamselae in sobaity sea bream (Sparidentex hasta) Fry. Turkish J. Fish. Aquat. Sci. 18(4): 633-645.

Jegede, T. 2012. Effect of garlic (Allium sativum) on growth, nutrient utilization, resistance and survival of Tilapia zillii (Gervais 1852) fingerlings. J. Agric. Sci. 4(2): 269.

Jeyakumari, A., Ninan, G., Joshy, C.G., Parvathy, U., Zynudheen, A.A. and Lalitha, K.V. 2016. Effect of chitosan on shelf life of restructured fish products from pangasius (Pangasianodonhypophthalmus) surimi during chilled storage. J. Food Sci. Technol.53(4): 2099-2107.

Kalyankar, A.D., Gupta, R.K., Bansal, N., Sabhlok, V.P. and Singh, D. 2013. Effect of garlic (Allium Sativum) against AeromonasHydrophila and health management of Swordtail, XiphophorusHelleri A.D. J. Environ. Sci. Sustainability. 1(2): 41–48.

Kaur, H. andAnsal, M.D. 2020. Efficacy of garlic powder as a growth promoting feed additive for higher growth and improved flesh quality in an Indian major carp, Labeorohita (Ham.) fingerlings. Methods. 9(13): 14.

Khalil, A.M., Yasuda, M., Farid, A.S., Desouky, M.I., Mohi-Eldin, M.M., Haridy, M. and Horii, Y. 2015. Immunomodulatory and antiparasitic effects of garlic extract on Eimeriavermiformis-infected mice. Parasitol. Res.114(7): 2735-2742.

Kim, S.R., Jung, Y.R., An, H.J., Kim, D.H., Jang, E.J., Choi, Y.J.and Chung, H.Y. 2013. Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-κBsignaling. PloS one. 8(9): e73877.https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073877

Kumar, A., Harikrishna, V., Reddy, A.K., Chadha, N.K. and Rani A.M.B. 2017. Salinity tolerance of Pangasianodonhypophthalmus in inland saline water: effect on growth, survival and haematological parameters. Eco. Env. & Cons.23 (1): 475-482.

Lee, J. Y., & Gao, Y. (2012). Review of the application of garlic, Allium sativum, in aquaculture. Journal of the World Aquaculture Society, 43, 447-458.

Lefevre, S., Huong, D.T.H., Nguyen, T.K., Wang, Nguyen, T.P. and Bayley, M. 2011. A telemetry study of swimming depth and oxygen level in a pangasius pond in the Mekong Delta. Aquaculture, 315(3-4): 410-413.

Lewis, W.H. and Elvin-Lewis, M.P.F. 2003. Medical Botany: Plants Affecting Human Health. Edn 2. New York, Wiley, 832

Naveenkumar, B.T., Kaur, Y., Tyagi, A.and Shanthanagouda, A.H. 2017. Baseline studies on selected hematological parameters of Indian major carps, exotic carps and catfishes. J. Fish Life Sci. 2: 60-67.

Ndong, D. & Fall, J., (2007). The effect of garlic (Allium sativum) on growth and immune responses of hybrid tilapia (Oreochromis niloticus x Oreochromis aureus). J. Clin. Immunol. Immunopathol. Res., 3(1): 1-9

Nwabueze, A.A.2012. The effect of garlic (Allium sativum) on growth and haematological parameters of Clariasgariepinus (Burchell, 1822). Sustain. Agric. Res.1(526-2016-37830).

Paulraj, R. andEasterson, D.C.V. 1982. Determination of digestibility coefficient. CMFRI special publication-Manual of Research Methods for Fish and Shellfish Nutrition, (8): 75-81.

Patel, P.P., Patel, M.P. and Tank, P.R. 2019. Effect of Herbal Liver Tonic on the Growth, Digestibility and Bio-chemical Composition of Grey Mullet Mugilcephalus (Linnaeus) fry. Int. J. Curr. Microbiol. App. Sci. 7(8): 2741-2749.

PrayogiSunu, D.S., Mahfudz, L.D. andYunianto, V.D. 2019. Prebiotic activity of garlic (Allium sativum) extract on Lactobacillus acidophilus. Vet. World. 12(12): 2046.

Rafiee, G.R., Saad, C.R., Kamarudin, M.S., Ismail, M.R. and Alavi, S.M.H. 2006. Estimation of ammonia excretion rates during a period of red tilapia, Oreochromis sp. Culture, considering biomass increase in a water recirculating system. Indian J.Fish.6(1): 69-82.

Rahman, K. 2003. Garlic and aging: a new insight into an old remedy. Ageing Res. Rev. 2:39-56.

Rao, G.K. 2010. Present status of pangasius, Pangasianodonhypophthalmus (Sauvage) farming in Andhra Pradesh, India. A comprehensive study report, submitted to the National Fisheries Development Board (NFDB), Hyderabad, India, pp: 41

Saleh, N.E., Michael, F.R. and Toutou, M.M. 2020. Evaluation of garlic and onion powder as phyto-additives in the diet of sea bass (Dicentrarcuslabrax). Egypt. J. Aquat. Res. 41(2): 211-217.

Sawant, G.P., Singh, H., Sawant, N.H. and Yusufzai, S.I. (2003). Bioenergetic studies on the fry of Liza parsia (Hamilton- Buchanan, 1822) using three different oil cakes. Indian J. Applied and Pure Bio. 18(2):169-178.

Setijaningsih, L., Setiadi, E. and Taufik, I. 2021, April. The effect of garlic Allium sativum addition in feed to the growth performance and immune response of tilapia Oreochromisniloticus. In IOP Conference Series: Earth and Environmental Science. 744(1): 012072 I OP Publishing.

Shalaby, A.M., Khattab, Y.A. and Abdel Rahman, A.M. 2006. Effect of Garlic (Allium sativum) and Chloramphenicol on growth performance, physiological parameters and survival of Nile Tilapia (Oreochromisniloticus). J. Venom. Anim. Toxins incl. Trop. Dis. 12(2): 172-201.

Shrestha, M.K., Pandit, N.P., Bista, J.D. and Dahal, S.P. 2015. Polyculture of mixed-sex Nile tilapia (Oreochromisniloticus) with pangas catfish (Pangasiushypophthalmus). Nepalese J.Aqua.Fish.2: 86-93.

Silarudee, S., Tongpim, S., Charoensri, N. and Doolgindachbaporn, S. 2019. Effect of a Probiotic Lactobacillus plantarum CR1t5 dietary supplements on non-specific immunity in black eared catfish (Pangasiuslarnaudii). J. Pure Appl. Microbiol. 13(1): 289-296.

Talpur, A.D. and Ikhwanuddin, M.H.D. 2012. Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Latescalcarifer (Bloch). Aquaculture. 364: 6-12.

Tu-Tran, L.C., Nguyen, T.C., Verreth, J.A. andSchrama, J.W. 2020. Doses response of dietary viscosity on digestibility and faecal characteristics of striped catfish (Pangasionodonhypophthalmus). Aquac. Res.51(2): 595-604.

Volstorf, J. 2021. Pangasianodonhypophthalmus (Findings). In: Fish Etho Base, ed. Fish Ethology and Welfare Group. World Wide Web electronic publication. Version 1. 327. www.fishethobase.net.

Wintrobe, M.M. 2008. Wintrobe's clinical hematology (Vol. 1). Lippincott Williams & Wilkins.

Xu, A., Shang‐Guan, J., Li, Z., Gao, Z., Huang, Y. and Chen, Q. 2020. Effects of garlic powder on feeding attraction activity, growth and digestive enzyme activities of Japanese seabass, Lateolabraxjaponicus. Aquac. Nutr.26(2): 390-399.

Yigit, M., Koshio, S., Aral, O., Karaali, B.andKarayucel, S. 2003. Ammonia nitrogen excretion rate-An index for evaluating protein quality of three feed fishes for the black sea turbot.Isr. J. Aquac.–Bamidgeh. 55(1): 69-76

Zaefarian, A., Yeganeh, S. andAdhami, B. 2017. Dietary effects of garlic powder (Allium sativum) on growth, blood indices, carcass composition, and lysozyme activity in brown trout (Salmocaspius) and resistance against Yersinia ruckeri infection. Aquac. Int. 25(6): 1987-1996.

Zare, M., Tran, H.Q., Prokesova, M. and Stejskal, V. 2021. Effects of Garlic Allium sativum powder on nutrient digestibility, haematology, and Immune and stress responses in Eurasian Perch Percafluviatilis Juveniles. Animals. 11(9): 2735.

Zewdie, A.K. 2019. The different methods of measuring feed digestibility: EC Nutr. 14(1): 68-74.

Downloads

Submitted

13-11-2022

Published

15-03-2023

Issue

Section

Non-Ruminants

How to Cite

Patel, P., R.V.Borichangar, Patel, V. R., & Timbadia, S. (2023). Garlic Powder Supplementation: Effects on Nutrient Utilization and Haematological Parameters of Sutchi catfish (Pangasianodon hypophthalmus, Sauvage, 1878). Indian Journal of Animal Nutrition, 39(4). https://epubs.icar.org.in/index.php/IJAN/article/view/130175