Effect of Inclusion of Hatchery Discarded Infertile Egg Meal with Shell on In Vitro Digestibility of Puppy Food

Abstract

The present study was undertaken to investigate the effect of inclusion of infertile egg meal with shell obtained from the hatchery n in vitro digestibility of Labrador puppy food. the infertile egg meal obtained from the GADVASU hatchery was first broken in a tray along with shell and dired in a hot air oven at 65^oC for 48 hours and after drying grinded to form infertile egg meal with shell (IEMS). The infertile egg meal was subsequently analysed for microbiological examination and in vitro digestibility of puppy food at graded level of inclusion of 2.5 %, 5 %, 7.5 % and 10 %. The result of chemical analysis of the IEMS revealed that the DM, CP, EE, CF, Ash, AIA, Ca and P was 35.55 %, 33.25 %, 26.50 %, , 0.1 %, 20.5 %,  0.1 %, , 9.1 %  and 1.13 %  respectively. The microbiological examination revealed that IEMS samples were negative for negative for salmonella and E. coli bacteria, while the total viable count was 2060 CFU at 1:10 dilution which subsequently become nil in further dilution means that bacterial contamination of IEMS is very minimal or negligible. Results revealed that digestibility of dry matter was significantly (P<0.05) highest in commercial puppy food (87.30 %) and 2.50 % level of IEMS (86.12 %) and was significantly (P<0.05) lowest at 10 % level of IEMS (82.94 %). The organic matter digestibility was significantly (P<0.05) higher in commercial puppy food (88.45 %) and 2.5 % IEMS level (87.44%) as compared to 7.5 % (84.85 %) and 10 % (84.05 %) level of inclusion of IEMS however, it was comparable with 5 % level of IEMS inclusion. The ether extract digestibility was significantly (P<0.05) highest in 2.5 % level of IEMS (97.07%) and significantly (P<0.05) lowest in commercial puppy feed (91.96 %). The crude protein digestibility was significantly (P<0.05) higher in the commercial puppy food, 2.5 %, 5 % and 7.5 % level of IEMS as compared to 10 % level of IEMS. So, it can be concluded from the present study that IEMS is a good source of alternative protein and can be included at 2.5 and 5 % level in the diet of Labrador puppy food.