Effect of Probiotic and Exogenous Fibrolytic Enzyme Treated Rice Straw on the Performance of Hariana Heifers


192 / 44

Authors

  • A.Patel
  • V. Kumar Department of Animal Nutrition College of Animal Science & Animal Husbandry, UP Pt Deen Dayal Upadhaya Veterinary University and Go Anu Sandhan Sansthan, (DUVASU), Mathura-281001, Uttar Pradesh, India
  • M. Kumar
  • R. Kushwaha
  • S. Vaswani
  • A. Kumar

Keywords:

Heifers, Probiotics, Exogenous fibrolytic enzyme, Paddy straw

Abstract

This study was conducted to evaluate the probiotic and exogenous fibrolytic enzyme treated rice straw on the performance of Heifers. Eighteen heifers were selected and allocated into three groups having six animals in each and fed treatment diet for 120 days. The control group was fed on basal diet whereas, group-1 was supplemented basal diet with 5g/kg probiotic (Saccharomyces cerevisiae) and group-2 was supplemented basal diet with 7g/kg exogenous fibrolytic enzymes (EFE). The nutrient requirements of heifers were met by feeding concentrate mixture, Jowar, and paddy straw (50:30:20 ratio) on DM basis. No effect on DMI, FCR, FCE but body weight gain was found significantly higher (P<0.05) in supplemented group. There was no significant effect (P<0.05) of supplementation on blood parameters except Hb and HCT levels which was significantly higher in the treatment groups. Treatment groups showed significantly higher (P<0.05) plasma total protein, plasma globulin, plasma cholesterol, plasma ALP and lower bilirubin whereas, no significant effect (P>0.05) was found on albumin, glucose, triglycerides, ALT, AST, creatinine and PUN levels. BHBA and IGF-1 were similar in all group but NEFA, FRAP and SOD were significantly different (P<0.05) in control, probiotic and EFE group, respectively.  There was no significant effect on plasma minerals except P and Se. there was no effect on animal behaviour parameters after supplementation but rumen enzyme i.e., cellulase, avicelase, and xylanase activity in EFE group showed significantly higher (P<0.05) than probiotic or control group. Finally, it may be concluded that probiotics (Saccharomyces cerevisiae) and exogenous fibrolytic enzyme @ 5 and 7 g per kg DM, respectively improved weight gain and digestibility of nutrients without affecting blood haematology, biochemical parameters, mineral profile and ingestive behaviour adversely. Thus, rice straw may be included in ration of ruminant up to 20% of DM with probiotics (Saccharomyces cerevisiae) and exogenous fibrolytic enzyme treatments.

Author Biography

  • V. Kumar , Department of Animal Nutrition College of Animal Science & Animal Husbandry, UP Pt Deen Dayal Upadhaya Veterinary University and Go Anu Sandhan Sansthan, (DUVASU), Mathura-281001, Uttar Pradesh, India

    Associate Professor

    Department of Animal Nutrition
    College of Animal Science & Animal Husbandry, UP Pt Deen Dayal Upadhaya Veterinary University and Go Anu Sandhan Sansthan, (DUVASU), Mathura-281001, Uttar Pradesh, India

References

AOAC, 2005. Official Methods of Analysis. 18th edn. Association of official Analytical Chemists, Virginia, USA.

Arjomand, M., Nobakht, A., Mehmannavaz, Y. 2022. Effects of Different Forms of Cattle Colostrum for Broiler Chickens. Iranian Journal of Applied Animal Science.12:371-378.

Arriola, Kim, K.G., Staples, S.C., Adesogan, C.R. 2011. Effect of fibrolytic enzyme application to low- and high-concentrate diets on the performance of lactating dairy cattle. Journal of Dairy Science. 94: 832-841.

Avellaneda, Y., Yaich, H., Malek, A., Rekhis, J., Kamoun, M. 2009. Influence of adding fibrolytic enzymes on the ruminal fermentation of date palm by-products. Archives Animal Breeding. 62:1-8.

Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology. 299: 15-27.

Bhasker, T.V., Nagalakshmi, D., Srinivasa Rao D, Raghunandan, T. 2013. Effect of supplementing exogenous fibrolytic enzyme cocktail on nutrient utilization in sheep fed on maize stover based total mixed ration. Indian Journal of Animal Nutrition. 30: 47-51.

Bowman, Beauchemin, G.R. and Shelford, K.A. 2002. The proportion of the diet to which fibrolytic enzymes are added affects nutrient digestion by lactating dairy cows. Journal of Dairy Science. 85:3420-3429.

Chandrasekhar, P., Ramachandra, B., Suresh, B.N., Madhav Prasad, C.B. and Shetter, V.B. 2009. Effect of complete diet with or without yeast culture in sheep. Indian Journal of Animal Nutrition. 27:81-84.

Dean, D.B., Staples, C.R., Littell, R.C., Kim, S. and Adesogan, A.T. 2013. Effect of method of adding a fibrolytic enzyme to dairy cow diets on feed intake digestibility, milk production, ruminal fermentation, and blood metabolites. Animal Nutrition and Feed Technology. 13: 337-353.

Erasmus, L.J., Botha, P.M. and Kistner, A. 1992. Effect of yeast culture supplement on production, rumen fermentation, and duodenal nitrogen flow in dairy cows. Journal of Dairy Science. 75(11): 3056-3065.

Forsberg, C.W.V., Beveridge, T.J. and Hellstrom, A. 1981. Cellulase and xylanase release from Bacteroides succinogenes and its importance in the rumen environment. Applied Environ Microbiology, 42:886-895.

Gado, H.M., Salem, A.Z.M., Camacho, L.M., Camacho, L.M., Elghandour, M.M.Y. and Salazar, M.C. 2013. Influence of exogenous enzymes on in vitro ruminal degradation of ensiled rice straw with DDGS. Animal Nutrition and Feed Technology. 13: 569-574.

Ganai, A.M., Matoo, F.A., Singh, P.K., Ahmad, H.A., Samoon, M.H. 2006. Chemical composition of some feeds, fodders, and plane nutrition of livestock of Kashmir valley. SKUAST Journal of Research. 8:145-151.

Giang, V.D. and Trach, N.X. 2020. Effects of treatments with lime and/or urea on rice straw chemical composition, intake, and degradability. Animal Feeding in Vietnam, (PAFVʼ02), The Agricultural Publishing House, Hanoi, Vietnam, 78:162-175.

Giraldo, L.A., Ranilla, M.J., Tejido, M.L. and Carro, M.D. 2014. Effects of exogenous fibrolytic enzymes on in vitro ruminal fermentation of substrates with different forage: concentrate ratios. Animal Feed Science and Technology, 141:306-325.

Guedes, C.M., Goncalves, D., Rodrigues, M.A.M., Dias-da-Silva, A. 2018. Effects of a Saccharomyces cerevisiae yeast on ruminal fermentation and fiber degradation of rice straw in cows. Animal Feed Science and Technology. 145:27-40.

Hosoda, K., Kurumoto, K., Eruden, B., Nishida ,T. and Shioya, S. 2006. The effect of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in Holstein steers. Asian Australasian Journal of Animal Sciences, 19(1): 35-41.

Jouany, J.P. and Morgavi, D.P. 2007. Use of ‘natural’ products as alternatives to antibiotic feed additives in ruminant production. Journal of Animal Science. 1: 1443-1466.

Kander, M. 2004. Effect of Bifidobacterium sp. on the health state of piglets, determined on the basis of hematological and biochemical indices Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine, 4:321-326.

Kholif, A.E., Hamdon, H.A., Gouda, G.A., Kassab, A.Y., Morsy, T.A. and Patra, A.K., 2022. Feeding Date-Palm Leaves Ensiled with Fibrolytic Enzymes or Multi-Species Probiotics to Farafra Ewes: Intake, Digestibility, Ruminal Fermentation, Blood Chemistry, Milk Production and Milk Fatty Acid Profile. Animals 12, 1107. https://doi.org/10.3390/ ani12091107 accessed 20/11/2023.

Madesh, M. and Balasubramanian, K.A. 1998. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal Biochemistry and Biophysics. 35(3): 184-188.

Magaye Ruth, R., Yue, X., Zou, B., Shi, H., Yu, Y., Liu, K., Lin, X., Xu, J., Yang, C., Wu, A. and Zhao, J. 2014. Effect of supplementation of yeast culture to calves fed with a complete diet. Indian Veterinary Journal. 87: 667-669.

Malik, R. and Srinivas, B. 2010. Effect of source and dose of probiotics and exogenous fibrolytic enzymes (EFE) on intake, feed efficiency, and growth of male buffalo (Bubalus bubalis) calves. Tropical Animal Health and Production. 42(6): 1263- 1269.

Milewski, S. and Sobiech, P. 2009. Effect of dietary supplementation with Saccharomyces cerevisiae dried yeast on milk yield, blood biochemical and hematological indices in ewes. Indian Journal of Animal Nutrition. 53:753-758.

National Research Council. 2001. Nutrient Requirements of Dairy Cattle. 7th revision Edition, The National Academic Press, Washington, DC, USA.

Peters, A., Meyer, U. and Danicke, S. 2015. Effect of exogenous fibrolytic enzymes on performance and blood profile in early and mid-lactation Holstein cows. Animal Nutrition, 1:229-238.

Pettersson, V., Barcellos, J.O., Prates, E.R., Mcmanus, C., Silva, L.P., Stella, L.A., Lopes, R.B. 2008. Nutritional value of baled rice straw for ruminant feed. Journal Dairy Science, 45(7):392-399.

Refat B, 2018. Molecular structure features and nutrient availability and utilization of barley silage varieties with varying digestible structural carbohydrate in comparison with a new short-season corn silage in high-producing dairy cattle. PhD Thesis. University of Saskatchewan, Saskatoon, Canada.

Salawu, M.B., Acamovic, T., Stewart, C.S., Hvelplund, T. and Weisbjerg, M.R. 1999. The use of tannins as silage additives: effects on silage composition and mobile bag disappearance of dry matter and protein. Animal Feed Science and Technology. 82:243-259.

Sharma, A.N., Kumar, S. and Tyagi, A.K. 2018. Effects of mannan-oligosaccharides and Lactobacillus acidophilus supplementation on growth performance, nutrient utilization and fecal characteristics in Murrah buffalo calves. Journal of Animal Physiology and Animal Nutrition. 102:679-689.

Sheikh, G.G., Ganai, A.M., Ishfaq, A., Afzal, Y. and Ahmad, H.A. 2017. In vitro effect of probiotic mix and fibrolytic enzyme mixture on digestibility of paddy straw. Advance Animal of Veterinary Science. 5:260-266.

Singh, A., Eruden, B., Nishida, T. and Shioya, S. 2018. Effect of feed additive supplementation on antioxidant status, immune characteristics, and energy and lipid metabolism in growing cattle. Biological Trace Element Research. 190: 65-75.

Titi, H.H. and Tabbaa. 2004. Dry chemical processing and ensiling of rice straw to improve its quality for use as ruminant feed. Tropical Animal Health and Production, 45: 1215-1221.

Van Soest, P.J., Robertson, J.B. and Lewis, B.A. 1991. Methods for dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. Journal of Dairy Sciences. 74: 3583-3597.

Van Soest PJ. 2006. Rice straw, the role of silica and treatments to improve quality. Animal Feed Science and Technology. 130: 137-171.

Ye, I.K. and Stern, M.D. 2009. Influence of direct-fed microbials on ruminal microbial fermentation and performance of ruminants: A review- Asia-Australasian Journal of Animal Science, enzymes on in vitro ruminal fermentation and milk. Asian-Australian Journal of Animal Science, 8 :533-555.

Zadrazil, F., Puniya, A. and Singh, K. 1995. Biological upgrading of feed and feed components. In: Biotechnology in Animal Feed and Feeding. R J Wallace and A Chesson (eds), VCH Publishers, Weinheim. 1995: 57-70.

Zhuang Z, Xu D, Wang L, Hao J, Wang J, Zhou X. 2020. Effects of exogenous fibrolytic enzyme supplementation to diets with different concentrate-to-forage ratios on the performance, blood metabolites, and ruminal fermentation of lactating Holstein dairy cows. Journal of Dairy Science. 30 (2):126-132.

Downloads

Submitted

20-11-2023

Published

03-06-2024

Issue

Section

Ruminant

How to Cite

A.Patel, , V. K., M. Kumar, R. Kushwaha, S. Vaswani, & A. Kumar. (2024). Effect of Probiotic and Exogenous Fibrolytic Enzyme Treated Rice Straw on the Performance of Hariana Heifers. Indian Journal of Animal Nutrition, 41(1). https://epubs.icar.org.in/index.php/IJAN/article/view/145465