Effect of Various Levels of Exogenous Fibrolytic Enzymes and Fumaric Acid on the Digestibility, Methane Emission and Performance of Growing Sahiwal Calves


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Authors

Keywords:

Methane, exogenous fibrolytic enzymes, cellulase, xylanase, fumaric acid Sahiwal calves, growth trial, in vivo, Digestibility, organic acid

Abstract

This study was aimed to test whether combination of exogenous fibrolytic enzymes and fumarate as fumaric acid, a hydrogen sink may result in any complementary effects in vivo. Growing Sahiwal calves (18; 6–12 month-old; average body weight, 132 kg; range 79–192 kg) were arranged into 3 groups in a randomized complete block design. Treatments were: Control (no additives), T1 (control + 0.75 g/kg DM of EFE + Fumaric acid (FA) @ 2% of DM and T3 (control + 1.5 g/kg DM of EFE + FA @ 2% of DM). Exogenous fibrolytic enzymes (EFE) (cellulase with activity >4500 – 5000 µM glucose/g/min and Xylanase with activity >7500 – 8000 µM xylose/g/min, mixed in 50:50 w/w). During growth trial of 120 days, calves were fed with a basal diet containing sorghum stover, green grass (freshly cut) and concentrate mixture (50: 10: 40) ad lib. (15% in excess of the previous day’s intake). Various levels or doses of EFE in combination with FA had no effect on final body weight, average daily gain and daily feed intake. There was no effect of supplementation of additives on apparent digestibility of DM, OM, CF, EE, NDF, and ADF but CP digestibility increased as a result of T1 treatment. Nitrogen intake and nitrogen retention was similar in three groups. Methane energy estimated as loss of GE intake decreased by 13.54 and 10.76 per cent, for T1 and T2 respectively, compared to the control and methane expressed as g/kg digestible dry matter intake was 12.29% lower on T1 treatment and 5.03 % with T2 compared to the control. These reductions were not reflected in growth performance of calves. It can be concluded that reduction of methane production caused by feed additives to the basal diet in the present study was though quantifiable it could not improve animal performance and nutrients utilization in contrast to the previous in vitro findings observed during the use of EFE and organic acids combination on roughage based diet.

Author Biography

  • Sudheer Babu Arumbaka, PVNR TVU, Hyderabad-30

    A. Sudheer Babu,

    Assistant Professor,

    Department Of Animal Nutrition,

    College of Veterinary Science,

    Rajendranagar, Hyderabad -500030

    Telangana State

    Cell: 8008242792

    bakasudheer@gmail.com

References

Abdl-Rahman, M.A., Sawiress, F.A.R. and Abd El-Aty, A.M., 2010. Effect of Sodium Lauryl Sulfate‐Fumaric Acid Coupled Addition on the In Vitro Rumen Fermentation with Special Regard to Methanogenesis. Veterinary Medicine International, 2010(1), 858474.

Alvarez, G., Pinos-Rodriguez, J.M., Herrera, J.G., Garcia, J.C., Gonzalez, S.S. and Barcena, R., 2009. Effects of exogenous fibrolytic enzymes on ruminal digestibility in steers fed high fiber rations. Livestock Science, 121(2-3), 150-154.

AOAC, 2005. Official Methods of Analysis. 18th Edn. Association of official Analytical Chemists, Virginia, USA.

Arriola, K.G., Kim, S.C., Staples, C.R. and Adesogan, A.T., 2011. Effect of fibrolytic enzyme application to low-and high-concentrate diets on the performance of lactating dairy cattle. Journal of Dairy Science, 94(2), 832-841.

Bayaru, E., Kanda, S., Kamada, T., ITABASHI, H., ANDOH, S., NISHIDA, T., ISHIDA, M., ITOH, T., NAGARA, K. and ISOBE, Y., 2001. Effect of fumaric acid on methane production, rumen fermentation and digestibility of cattle fed roughage alone. Nihon Chikusan Gakkaiho, 72(2), 139-146.

Beauchemin, K.A., Kreuzer, M., O’mara, F. and McAllister, T.A., 2008. Nutritional management for enteric methane abatement: a review. Australian Journal of Experimental Agriculture, 48(2), 21-27.

Beauchemin, K.A., Colombatto, D., Morgavi, D.P. and Yang, W.Z., 2003. Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science, 81(14_suppl_2), E37-E47.

Blummel, M., Rao, S.S., Palaniswami, S., Shah, L. and Reddy, B.V., 2009. Evaluation of sweet sorghum (Sorghum bicolor L. Moench) used for bio-ethonol production in the context of optimizing whole plant utilization. Animal Nutrition and Feed Technology, 9(1), 1-10.

Carro, M.D., Ranilla, M.J., Giráldez, F.J. and Mantecón, A.R., 2006. Effects of malate on diet digestibility, microbial protein synthesis, plasma metabolites, and performance of growing lambs fed a high-concentrate diet. Journal of animal science. 84(2), 405-410.

Chhabra, A., Manjunath, K.R., Panigrahy, S. and Parihar, J.S., 2009. Spatial pattern of methane emissions from Indian livestock. Current Science. 683-689.

Chung, Y.H., Zhou, M., Holtshausen, L., Alexander, T.W., McAllister, T.A., Guan, L.L., Oba, M. and Beauchemin, K.A., 2012. A fibrolytic enzyme additive for lactating Holstein cow diets: ruminal fermentation, rumen microbial populations, and enteric methane emissions. Journal of dairy science, 95(3) 1419-1427.

Dean, D.B., Staples, C.R., Littell, R.C., Kim, S. and Adesogan, A.T., 2013. Effect of method of adding a fibrolytic enzyme to dairy cow diets on feed intake digestibility, milk production, ruminal fermentation, and blood metabolites. Animal Nutrition and Feed Technology, 13(3).337-353.

Dong, Y., Bae, H.D., McAllister, T.A., Mathison, G.W. and Cheng, K.J., 1999. Effects of exogenous fibrolytic enzymes, α-bromoethanesulfonate and monensin on fermentation in a rumen simulation (RUSITEC) system. Canadian Journal of Animal Science. 79(4).491-498.

Eun, J.S., ZoBell, D.R., Dschaak, C.M. and Diaz, D.E. 2008. Effect of a fibrolytic enzyme supplementation on growing beef steers. Proceedings, Western Section, American Society of Animal Science, 59: 418-420.

Foley, P.A., Kenny, D.A., Callan, J.J., Boland, T.M. and O'Mara, F.P., 2009. Effect of DL-malic acid supplementation on feed intake, methane emission, and rumen fermentation in beef cattle. Journal of animal science. 87(3).1048-1057.

Forster, P., Ramaswamy, V., Artaxo, P., Berntsen, T., Betts, R., Fahey, D.W., Haywood, J., Lean, J., Lowe, D.C., Myhre, G., Nganga, J., Prinn, R., Raga, G., Schulz, M., Van Dorland, R., 2007. Changes in Atmospheric Constituents and in Radiative Forcing. Cambridge University Press, Cambridge, United Kingdom.

Giraldo, L.A., Ranilla, M.J., Tejido, M.L. and Carro, M.D., 2007. Influence of exogenous fibrolytic enzymes and fumarate on methane production, microbial growth and fermentation in Rusitec fermenters. British Journal of Nutrition. 98(4).753-761.

Giraldo, L.A., Tejido, M.L., Ranilla, M.J., Ramos, S. and Carro, M.D., 2008. Influence of direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet. Journal of animal science. 86(7).1617-1623.

Holtshausen, L., Chung, Y.H., Gerardo-Cuervo, H., Oba, M. and Beauchemin, K.A. 2011. Improved milk production efficiency in early lactation dairy cattle with dietary addition of a developmental fibrolytic enzyme additive. Journal of Dairy Science, 94: 899-907.

IAEA,1997. Estimation of rumen microbial protein production from purine derivatives in urine. International Atomic Energy Agency TECDOC-945, pp. 22–24. Vienna.

Johnson, K., Huyler, M., Westberg, H., Lamb, B. and Zimmerman, P., 1994. Measurement of methane emissions from ruminant livestock using a sulfur hexafluoride tracer technique. Environmental science & technology. 28(2).359-362.

Johnson, K.A., Johnson, D.E., 1995. Methane emissions from cattle. Journal of animal science. 73, 2483-2492.

Kolver ES, Aspin PW (2006) Supplemental fumarate did not infl uence milk solids or methane production from dairy cows fed high quality pasture. Proceedings of New Zealand Society of Animal Production 66:409–415

Kolver ES, Aspin PW, Jarvis GN (2004) Fumarate reduces methane production from pasture fermented in continuous culture. Proceedings of New Zealand Society of Animal Production 64:155–159

Li, X.Z., Long, R.J., Yan, C.G., Lee, H.G., Kim, Y.J. and Song, M.K., 2011. Rumen microbial response in production of CLA and methane to safflower oil in association with fish oil or/and fumarate. Animal science journal, 82(3).441-450.

Lin, B., Wang, J.H., Lu, Y., Liang, Q. and Liu, J.X., 2013. In vitro rumen fermentation and methane production are influenced by active components of essential oils combined with fumarate. Journal of Animal Physiology and Animal Nutrition. 97(1).1-9.

Liu Q, Wang C, Yang WZ et al (2009) Effects of malic acid on rumen fermentation, urinary excretion of purine derivatives and feed digestibility in steers. Animal 2:32–39

Martin, S., Streeter, M., Nisbet, D., Hill, G., Williams, S., 1999. Effects of dl-malate on ruminal metabolism and performance of cattle fed high-concentrate diet. Journal of Animal Science.77, 1008–1015.

Mohini M, Singhal K K, Sirohi S S and Mohanta R K. 2009. Methane emission from Sahiwal cows on dieatary supplementation of fumaric acid as a feed additives. Indian Journal of Animal Nutrition. 26(1): 56–60.

Morgavi D P Beauchemin K A Nsereko V L and Rode L M 2000 . Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum in degrading fiber substrates. Journal of Dairy Science. 83: 1310-1321.

Patra, A. K. (2016). Recent advances in measurement and dietary mitigation of enteric methane emissions in ruminants. Frontiers in veterinary science, 3.

Sniffen, C.J., Ballard, C.S., Carter, M.P., Cotanch, K.W., Dann, H.M., Grant, R.J., Mandebvu, P., Suekawa, M. and Martin, S.A., 2006. Effects of malic acid on microbial efficiency and metabolism in continuous culture of rumen contents and on performance of mid-lactation dairy cows. Animal feed science and technology. 127(1-2).13-31.

Sudheer Babu, A. 2017. Evaluation of urea treated stover supplemented with fibrolytic enzymes and methane inhibitors in cattle calves. Ph.D. Thesis submitted to NDRI, Karnal.

Sutton, J.D., Phipps, R.H., Beever, D.E., Humphries, D.J., Hartnell, G.F., Vicini, J.L. and Hard, D.L. 2003. Effect of method of application of a fibrolytic enzyme product on digestive processes and milk production in holstein-Friesian cows. Journal of Dairy Science. 86: 546-556.

Tomar SK, Shete SM, Singh B (2010) Synchronization of ruminal energy and nitrogen supply to improve the ruminant productivity. Indian Journal of Animal Nutrition.27:327-338

Van Soest P J Robertson J B and Lewis B A1991 Methods of dietary fiber, neutral detergent fiber and non- starch polysaccharides in relation to animal nutrition. Journal of Dairy Science.74: 3583-3597.

Wood, T.A., Wallace, R.J., Rowe, A., Price, J., Yáñez-Ruiz, D.R., Murray, P. and Newbold, C.J., 2009. Encapsulated fumaric acid as a feed ingredient to decrease ruminal methane emissions. Animal Feed Science and Technology.152(1-2).62-71.

Yang, W. Z., Beauchemin, K. A and Rode, L .M. 2000. A comparison of methods of adding fibrolytic enzymes to lactating cow diets. Journal of Dairy Science. 83: 2512-2520

Zhou, M., Chung, Y.H., Beauchemin, K.A., Holtshausen, L., Oba, M., McAllister, T.A. and Guan, L.L., 2011. Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive. Journal of applied microbiology, 111(5), pp.1148-1158.

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Submitted

16-06-2024

Published

18-01-2025

Issue

Section

Ruminant

How to Cite

Arumbaka, S. B., Madhu Mohini, S. S. Thakur, Goutam Mondal, Sujata Pandita, Manju Ashutosh, & B .S. Chandel. (2025). Effect of Various Levels of Exogenous Fibrolytic Enzymes and Fumaric Acid on the Digestibility, Methane Emission and Performance of Growing Sahiwal Calves. Indian Journal of Animal Nutrition, 41(3). https://epubs.icar.org.in/index.php/IJAN/article/view/152826