Nutritional and Microbiological Evaluation and in vitro Digestibility of Two In-House Formulated Feed for Adult Dogs
108 / 4
Keywords:
Dog feed, Feed formulation, in vitro digestibility, Microbiological evaluationAbstract
This study was conducted to develop an alternative feed option, addressing the demand for traditional extruded adult dog feed and exploring the possibilities of the development of vegetarian dog feed. Two types of extruded dog feed were formulated, namely a vegetarian variant (T1) and a non-vegetarian one (T2). The formulations were further subjected to a comparative study along with commercially available dog feed T3. On microbiological evaluation, both formulations revealed a total viable count of 2030 CFU at 10-1 dilution, which became negligible in further dilutions, indicating minimal bacterial contamination. The results of in vitro digestibility study demonstrated that the digestibility of dry matter and crude protein was significant (P ≥0.05) higher in T1 and T2 group in which in house prepared vegetarian and non-vegetarian food was fed as compared to T3 group in which commercial food was fed, however, the digestibility of ether extract was significantly higher in T1 and T3 group as compared to T2. Moreover, the digestibility of organic matter was significantly lower in T2 group then T1 group but the digestibility of organic matter remains comparable in commercial food with vegetarian (T1) and non-vegetarian (T2) food. These findings highlight that in housed prepared vegetarian and non-vegetarian were equal good to commercial dog food in term of nutritional quality and safety.
References
AAFCO. 2014. Official Publication. Champaign, IL: Association of American Feed control Officials.
Annex, I. 2010. Report of the Tenth Meeting of the OIE Animal Production Food Safety Working Group. World Organization for Animal Health: Paris, France.
AOAC. 2005. Official Methods of Analysis, 18th edition. Association of Official Analytical Chemists, Arlington, Virginia, USA.
Behravesh, C. B., Ferraro, A., and Deasy, M. 2010. Human Salmonella infections linked to contaminated dry dog and cat feed, 2006–2008. Pediatrics. 126:477– 483.
Biagi, G., Cipollini, I., Grandi, M., Pinna, C., Vecchiato, C. G., and Zaghini, G. 2016. A new in vitro method to evaluate digestibility of commercial diets for dogs. Italian Journal of Animal Science. 15(4):617-625.
Duncan, D.B. 1995. Multiple range and multiple F-test. Biometrica. 11:1-42.
Feed and Drug Administration. 2019. Sec. 683.100 Action Levels for Aflatoxins in Animal Feed https://www.fda.gov/ICECI/ Compliance Manuals /Compliance Policy Guidance Manual/default.htm)
Gazzotti, T., Biagi G, Pagliuca G, Pinna C, Scardilli M, Grandi, M. and Zaghini G. 2015. Occurrence of mycotoxins in extruded commercial dog feed. Animal Feed Science and Technology. 202: 81–89.
Girio, T. M. S., Nader Filho, A., Junior, O. R., Amaral, L. A. and Girio, R. J. S. 2012. Microbiological quality of dog feed sold in sealed packages and in bulk. Ars Veterinaria. 28:36–40.
Hołda, K., Głogowski, R., Hac-Szymańczuk, E., and Wiczuk, W. 2017. Comprehensive microbiological evaluation of dry feeds for growing dogs marketed in Poland. Ann Wars Univ Life Sci - SGGW Animal Science. 56:81–89.
Imanishi, M., Rotstein, D.S., Reimschuessel, R., Schwensohn, C.A., Woody, D.H., Davis, S.W., Hunt, A.D., Arends, K.D., Achen, M., Cui, J. and Zhang, Y. 2014. Outbreak of Salmonella enterica serotype Infantis infection in humans linked to dry dog food in the United States and Canada, 2012. Journal of the American Veterinary Medical Association. 244(5):545-553.
International Organization for Standardization. 2006. ISO 4832:2006, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coliforms Colony-count technique (3rd ed.). International Organization for Standardization.
IPFM. 2022. India pet feed market growth trends and forecast 2022-2027. (www.mordorintelligence.com)
Jansen, W., Mueller, A., Grabowski, N. T., Kehrenberg, C., Muylkens, B., and Al Dahouk, S. 2019. Foodborne diseases do not respect borders: Zoonotic pathogens and antimicrobial resistant bacteria in food products of animal origin illegally imported into the European Union. The Veterinary Journal. 244: 75-82.
Kaur, A., Sethi, A.P.S. and Singh, U. (2021). Comparative in-vitro evaluation of dog food using either rice gluten or maize fibre with commercially available dog food. Journal of Animal Research. 11(05): 01-06.
Kaur, M., Singh, U., Sethi, A.P.S., Kaur, H. and Malav, O.P. 2021a. Nutritional assessment of dal churi in dog food. Haryana Veterinarian. 60 (1): 119-122.
Kaur, M., Singh, U., Sethi, A.P.S., Hundal, J.S., Kaur, H. and Malav, O.P. 2021b. In vitro Nutritional Assessment of Soy Nuggets based Dog Food, Indian Journal of Animal Nutrition. 38(1): 86-91.
Kumar, S, Singh, U., Sharma, A., Lamba J.S., and Singh, Y. 2024. Evaluation of Chia Seeds in Dog Food. Indian Journal of Animal Nutrition. 41(1):163-168.
Leiva, A., Molina, A., Redondo-Solano, M., Artavia, G., Rojas-Bogantes, L. and Granados-Chinchilla, F. 2019. Pet food quality assurance and safety and quality assurance survey within the Costa Rican pet food industry. Animals. 9(11): 980.
Lupindu, A.M. 2017. Isolation and characterization of Escherichia coli from animals, humans, and environment. Escherichia Coli-Recent Advances on Physiology, Pathogenesis and Biotechnological Applications. London, United Kingdom: IntechOpen Limited. 187-206.
Manual, O.T. 2008. Equine Viral Arteritis.
National Research Council, Division on Earth, Life Studies, Committee on Animal Nutrition, Subcommittee on Dog, & Cat Nutrition. 2006. Nutrient requirements of dogs and cats. National Academies Press.
OIE. 2008 Salmonellosis-Chapter 2.9.9. In: OIE Terrestrial Manual. http://www.oie.int/fileadmin/Home/eng/Health_standards/ t a h m / 2 0 0 8 / p d f / 2.09.09_SALMONELLOSIS.pdf.
Ruzauskas, M., Virgailis, M., and Špakauskas, V. 2005. Serological diversity and antimicrobial resistance of Salmonella isolated from different sources in Lithuania. Veterinarski Arhiv. 75:211–221.
Sethi, A.P.S., Kaur, A. and Singh, U. 2019. Prospect and research priorities in canine nutrition. In lead paper presented at international conference on animal nutrition (INCAN 2019). “Nutritional strategies for improving farm profitability and clean animal production XVIII Biennial conference, Kolkata, West Bengal.
Singh, C., Sethi, A.P.S., Singh, U., Malav, O.P. and Kaur, H. 2020. Effect of Tomato Pomace supplementation on the nutritional value of dog diets as assessed by in- vitro digestibility. Journal of Animal Research: 10(6):925-930.
Singh, G., Uniyal, S., Sharma, A., Kaur, D. and Chahal. U.S. 2022. Effect of inclusion of hatchery discarded infertile egg meal with shell on in vitro digestibility of puppy food. Indian Journal of Animal nutrition. 39 (4): 480-485
Stull, J. W., Peregrine, A. S., Sargeant, J. M., and Weese, J. S. 2013. Pet husbandry and infection control practices related to zoonotic disease risks in Ontario, Canada. BMC Public Health. 13:520. doi: 10.1186/1471-2458-13-520
Suchodolski, J. S., Ruaux, C. G., Steiner, J. M., Fetz, K. and Williams, D. A. 2004. Application of molecular fingerprinting for qualitative assessment of small- intestinal bacterial diversity in dogs. Journal of Clinical Microbiology. 42:4702- 4708.
Talapatra, S.K., Ray, S.C., and Sen, K.C. 1940. The analysis of mineral constituents in biological materials. Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in feed-stuffs. Indian Journal of Veterinary Science. 10:243-258.
Tessari, E. N. C., Cardoso, A. L. S. P., Kanashiro, A. M. I., Stoppa, G. F. Z., Luciano, R. L. and de Castro, A. G. M. (2014). Analysis of the presence of Clostridium perfringens in feed and raw material used in poultry production. Food and Nutrition Sciences. 5:614–617, 2014.
Wojdat, E., Kwiatek, K., and Kozak, M. 2005. Microbiological quality of animal feeding stuffs in Poland. Bulletin of the Veterinary Institute in Pulawy. 49:315– 318.
Wojdat, E., Kwiatek, K., and Zasadny, R. 2004. Microbiological quality of pet feed in Poland. Polish Journal of Veterinary Sciences. 7:207–209.
Downloads
Submitted
Published
Issue
Section
License
Copyright remains with the society and author jointly. However, material can be used for research, teaching and to achieve goals of the society.