Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood


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Authors

  • Pallavi Baliga
  • Malathi Shekar
  • Shaik Tahur Ahamed
  • M. N. Venugopal

https://doi.org/10.21077/ijf.2019.66.2.86920-14

Abstract

Vibrio parahaemolyticus is a pathogen native to the aquatic environment. In this study, 46 environmental V. parahaemolyticus isolates were subjected to a correlational analysis to find the association between their antimicrobial susceptibility pattern, prevalence of CRISPR-Cas system and thermostable direct hemolysin (tdh) gene. Antibiotic resistance profiling against eleven antibiotics revealed the isolates to be multidrug resistant. Isolates exhibited highest resistance to vancomycin (97.8%) followed by ampicillin (91.3%), cefotaxime (69.6%), ceftazidime/clavulanic acid (54.4%), ceftazidime (45.7%) and gentamicin (39.1%). CRISPR loci and tdh gene were detected in 47.83 and 58.7% of strains respectively. No significant correlation was observed between antibiotic resistance to presence of CRISPR, except in the case of gentamicin wherein, a negative correlation was seen (r=-0.272, p<0.10). Similarly, tdh did not correlate to antibiotic resistance. Seventeen strains in this study harboured the CRISPR loci as well as tdh gene, the association of which was found to be statistically significant.

Keywords: Antimicrobial resistance, CRISPR, MAR index, Thermostable direct hemolysin (tdh), Vibrio parahaemolyticus

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Submitted

2019-02-13

Published

2019-06-30

Issue

Vol. 66 No. 2 (2019)

Section

Articles

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The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Baliga, P., Shekar, M., Ahamed, S. T., & Venugopal, M. N. (2019). Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood. Indian Journal of Fisheries, 66(2). https://doi.org/10.21077/ijf.2019.66.2.86920-14