Biochemical characterisation of lysozyme extracted from rainbow trout Oncorhynchus mykiss (Walbaum, 1792)


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Authors

  • Maryam Mohammad Gholipour MSc Student of Biochemistry, Department of Biology, Dezful Branch, Islamic Azad University, Dezful, Iran.
  • Abbass Moridnia PhD in Molecular Medicine, Department of Immunology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran.
  • Zahra Khoshnood Department of Biology, Dezful Branch, Islamic Azad University, Dezful, Iran. http://orcid.org/0000-0001-6340-6997

https://doi.org/10.21077/ijf.2021.68.2.96778-17

Abstract

In the present study, lysozyme was extracted from spleen tissue of rainbow trout Oncorhynchus mykiss (Walbaum, 1792)
and partially purified by ammonium sulfate precipitation. Optimum pH and temperature as well as effects of different salt
concentrations of NaCl, MgCl2, KCl and urea on enzyme activity were evaluated. The enzyme activity was assayed using a
suspension of Micrococcus lysodeikticus as substrate. Results showed that lysozyme extracted from the spleen of O. mykiss
has its optimum activity at high temperature (60ºC) and low pH (5.8) conditions and the enzyme activity was found to vary
with different salt concentrations which were found related to the environmental conditions of natural habitats of O. mykiss.
Keywords: Enzymology, Immune System, Lysozyme, Rainbow trout

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Submitted

2020-01-05

Published

2021-08-20

Issue

Vol. 68 No. 2 (2021)

Section

Note

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How to Cite

Mohammad Gholipour, M., Moridnia, A., & Khoshnood, Z. (2021). Biochemical characterisation of lysozyme extracted from rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Indian Journal of Fisheries, 68(2). https://doi.org/10.21077/ijf.2021.68.2.96778-17