Analysis of epigenetic chromatin modification during mitosis in rice
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Authors
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Rui Guo
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
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Chao Xue
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
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Shuai Liu
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
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Mingliang Zhang
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
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Zhiyun Gong
Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
Abstract
DNA methylation and histone modification are two important types of epigenetic modification. In this study, we analyzed the dynamic changes and chromosomal localizations of four different epigenetic modifications, DNA 5-MeC, H3K4me3, H3K9me2, and H3S10Ph, during mitosis in rice. The results showed that signals from DNA 5-MeC and H3K4me3 were distributed in the heterochromatin and euchromatin regions respectively, but no obvious signals from H3K9me2 were detected. There were no significant changes noticed in these three modification patterns at different phases during mitosis. However, the H3S10Ph, which was mainly distributed in the centromeric regions, showed dynamic changes during mitosis that occurred mainly at prophase and anaphase but not at interphase and telophase.