Simple DNA extraction method for SSR-PCR analysis from different tissues of Tapiscia sinensis, an endangered plant species


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Authors

  • Xiao-Jun Zhou School of Life Sciences, Luoyang Normal University, 71 Longmen Road, Luoyang, China
  • Rong-Shan Yan School of Life Sciences, Northwest University, 229 Taibai Bei Road, Xi’an, China
  • Ya-Nan Xu School of Life Sciences, Northwest University, 229 Taibai Bei Road, Xi’an, China
  • Da-Wei Zou School of Life Sciences, Northwest University, 229 Taibai Bei Road, Xi’an, China
  • Peng Zhao School of Life Sciences, Northwest University, 229 Taibai Bei Road, Xi’an, China
  • Wen-Zhe Liu School of Life Sciences, Northwest University, 229 Taibai Bei Road, Xi’an, China

Abstract

A simple and rapid method to process different tissues of Tapiscia sinensis for effective plant DNA extraction were established in the study. DNA quality and yield obtained from bark, leaves and seeds were evaluated by Eppendorf BioPhotometer and SSR-PCR analysis. The results indicated that extracted genomic DNA based on the established method was sufficient for SSR-PCR profiling of different tree tissues. Hence, the sample treatment and extraction method can be used for SSR-PCR applications in tree genetic and molecular analysis research.

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Submitted

2016-11-29

Published

2016-11-29

Issue

Vol. 75 No. 3 (2015): The Indian Journal of Genetics and Plant Breeding

Section

Articles

How to Cite

Zhou, X.-J., Yan, R.-S., Xu, Y.-N., Zou, D.-W., Zhao, P., & Liu, W.-Z. (2016). Simple DNA extraction method for SSR-PCR analysis from different tissues of Tapiscia sinensis, an endangered plant species. The Indian Journal of Genetics and Plant Breeding, 75(3). https://epubs.icar.org.in/index.php/IJGPB/article/view/63511