Selection of optimal q RT-PCR reference genes for gene expression studies in foxtail millet
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Authors
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Zhiyong Li
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Yanbin Zhu
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Nan Wang
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Hui Bai
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Li Dong
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Jianzhang Quan
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
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Zhiping Dong
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences; National Foxtail Millet Improvement Center; Minor Cereal Crops Laboratory of Hebei Province, 126 Road hengshan, 050035 Shijiazhuang, China
Abstract
Appropriate reference gene is very important for gene expression analysis by the quantitative RT-PCR (qRT-PCR), but no systematics research have been carried out in foxtail millet.In this study,the mRNA expression were investigated
by qRT-PCR for six candidate reference genes, 25S rRNA, 18S rRNA, 17S rRNA, GAPDH, ACTB and UBQ under three different experimental conditions including different stages of leaf rust, five plant organs and seven different foxtail
millet cultivars. The results obtained with GeNorm, NormFinder and BestKeeper software tools showed stable and uniform expression of 25S rRNA among different cultivars, 18S rRNA under the different conditions of leaf rust infection and 17S rRNA in different foxtail millet organs The list of reference genes confirmed in present study will be useful for genes expression analysis in foxtail millet
under different conditions.