Development of an ISSR-derived SCAR marker linked to apospory in buffel grass (Cenchrus ciliaris L.)
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Authors
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Krishna Kumar Dwivedi
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
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A. Radhakrishna
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
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S. Kumar
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
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M.K. Srivastava
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
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M.G. Gupta
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
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P. Kaushal
Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi 284 003
Abstract
Cenchrus ciliaris (buffel grass) is a valuable forage grass, reproducing through aposporous apomixis. Inter Simple Sequence Repeat (ISSR) markers converted to SCAR marker linked to apospory in this crop is reported. Out of twentyfive ISSR primers tested in DNA bulks from apomictic and sexual progenies,five primers detected polymorphism.The 1.1 kb amplicon derived from the primer ISSR-856 showed co-segregation with apomictic mode of reproduction. Accordingly, a pair of SCAR (Sequence Characterized Amplified Region) primers were designed which also
generated a 1.1kb amplicon (Apo-856) only in the apomictic plants. Importance of such molecular markers in genetics and breeding of C. ciliaris is discussed.
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Submitted
2016-12-01
Published
2016-12-01
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Articles
How to Cite
Dwivedi, K. K., Radhakrishna, A., Kumar, S., Srivastava, M., Gupta, M., & Kaushal, P. (2016). Development of an ISSR-derived SCAR marker linked to apospory in buffel grass (Cenchrus ciliaris L.). The Indian Journal of Genetics and Plant Breeding, 75(2). https://epubs.icar.org.in/index.php/IJGPB/article/view/63612