Sucrose specificTRAP markers as genus and species specific markers in Saccharum and Erianthus spp.

Abstract

Sucrose specific candidate genes were used to identify
species specific markers through Target Region
Amplification Polymorphism (TRAP) based on a study
involving nineteen Erianthus sp., Saccharum officinarum
and S. spontaneum clones. In this study, 12 primer
combinations comprising of the forward primers of four
sequences of sucrose metabolizing genes and reverse
primers of three arbitrary sequences were used to amplify
the DNA samples of the species clones. A total of 296 bands
within a size range of 38 bp to 1216 bp were detected. Out
of these, 256 were polymorphic (86.49%) and 10 out of 12
combinations revealed more than 80% polymorphism.There
were five Erianthus specificTRAP markers (SuSy + Arb3108,
SuSy + Arb3260, SuPS(a) + Arb1155, SuPS(a) + Arb2370 andSAI
+ Arb3107) that would help in identifying intergeneric hybrids
in nobilization process. Eight Saccharum specific markers
(SuSy+Arb2144, SuSy+Arb3135, SuPS(a)+Arb1141, SuPS(a)+
Arb1518,SuPS(a)+Arb1628, SuPS(a)+Arb2212,SuPS(a)+Arb2282
and SuPS(b)+Arb1492 would enable detecting Saccharum
genome during nobilization process especially when
Saccharum is used as paternal parent. A marker specific to
S. officinarum (SuSy+Arb1960) owes promise as a marker
linked to sucrose content, being present in all sucrose rich
clones belonging to S. officinarum and absent in low
sucrose forms of Erianthus and S. spontaneum.