Cloning and expression analysis of a novel Glutathione S-transferase gene, MsGST, from alfalfa (Medicago sativa)


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Authors

  • Jie An Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Peizhi Yang Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Yuman Cao Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Jincai Geng Grassland Management Station of Shaanxi Province, Xi’an, Shaanxi, 710016, China
  • Penghui Ren Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Zhang You Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Yafang Wang Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Zhiqiang Zhang College of Grassland Resources and Environment, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010000, China
  • Jieyu Yang Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China

Abstract

Glutathione S-transferases are important enzymes in
protecting cells by scavenging reactive oxygen species
induced by various stresses. In this study, a novel GST
gene, MsGST (KM044312), was cloned and characterized
from alfalfa. The open reading frame of MsGST contains
660bp nucleotides, encoding 219 amino acid residues.
Amino acid sequence alignment indicated that the deduced
MsGST protein was highly homologous to other plant tau
class GST sequences. According to amino acid phylogenetic
analysis, the MsGST gene was clustered into the same
branch with other legume plants. Real-time quantitative
PCR (qRT-PCR) revealed that the expression levels of
MsGST were up-regulated in both shoots and roots under
ABA treatment and various stresses, including salt, drought,
cold and heat stress. The effect of nodules on MsGST gene
expression indicated that the induction of MsGST
expression by abiotic stress is independent of rhizobium
symbiosis. In conclusion, the MsGST gene may be involved
in response to different abiotic stresses in alfalfa.

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Submitted

2017-08-24

Published

2017-08-26

Issue

Vol. 77 No. 3 (2017): The Indian Journal of Genetics and Plant Breeding

Section

Articles

How to Cite

An, J., Yang, P., Cao, Y., Geng, J., Ren, P., You, Z., Wang, Y., Zhang, Z., & Yang, J. (2017). Cloning and expression analysis of a novel Glutathione S-transferase gene, MsGST, from alfalfa (Medicago sativa). The Indian Journal of Genetics and Plant Breeding, 77(3). https://epubs.icar.org.in/index.php/IJGPB/article/view/73586