Genomic variation studies in durum wheat (Triticum turgidum ssp. durum) using CBDP, SCoT and ISSR markers

Abstract

In the present study three DNA marker systems viz., start
codon targeted (SCoT), CAAT box-derived polymorphism
(CBDP) and inter-simple sequence repeats (ISSR) markers
were used to detect the genetic diversity and relationship
among 17 durum wheat genotypes. The ISSR primers
generated 130 bands across the genotypes with an average
of 8.12 bands per primer, whereas CBDP and SCoT primers
created 66 and 99 polymorphic bands with an average of
5.5 and 7.07 bands per primer, respectively. Values of MI
and PIC obtained for all three marker systems indicated the
high efficiency of these markers to detect the genetic
variation in durum wheat. The results revealed that ISSR
markers with an average of 83.46% polymorphism was a
suitable marker system to detect genetic variation among
genetic materials. On the other hand, gene targeted markers
(SCoT and CBDP), similar to ISSR marker, were
comparatively less efficient techniques to estimate the
genetic variation. The present findings revealed that the
genetic analyses using gene targeted markers such as
CBDP and SCoT would be more useful for crop
improvement programs.