Analysis of chalcone synthase gene in chemically induced male sterility (CIMS) in wheat (Triticum aestivum L.)
AbstractChemically induced male sterility (CIMS) is one of the male
sterility system used for hybrid wheat production in China.
However, the mechanism of sterility remains unclear. The
expression of chalcone synthase (CHS) gene was assessed
in anthers to determine its role in fertility/sterility in wheat.
The expression levels of CHS gene was evaluated by realtime quantitative polymerase chain reaction (qPCR). qPCR
analysis revealed that the expression levels of CHS gene
were downregulated in CIMS. The sporopollenin contents
in anthers underwent a sharp decrease in 1376-CIMS.
Scanning electron microscopy observations revealed that
pollen wall formation was significantly altered in 1376-CIMS.
This phenomenon implied that CHS gene expression was
inhibited during the fertility transformation process and
was related to the transformation from fertility to sterility.
Moreover, results indicated that CHS plays an essential
role in conducting fertility in chemically interfered male
How to Cite
Analysis of chalcone synthase gene in chemically induced male sterility (CIMS) in wheat (Triticum aestivum L.). (2017). The Indian Journal of Genetics and Plant Breeding, 77(4). https://epubs.icar.org.in/index.php/IJGPB/article/view/76259