Evaluating the expression of Hv TIP2;3 and Hv TIP4;1 in barley genotypes under different levels of salinity stress

Authors

  • Robab Salami Department of Plant Breeding and Biotechnology‚ Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran
  • Seyed Abolghasem Mohammadi Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz‚ 51666, Iran
  • Sara Ghaffariyan Department Biology‚ Faculty of Science, University of Shahid Madani Azarbijan, Iran
  • Mohammad Moghaddam Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz‚ 51666, Iran

Abstract

Barley is the most salt tolerant cereal and is grown in a
wide range of climatic conditions. To improve the plant
tolerance to salinity‚ expression analysis of genes involved
in stress tolerance could be effective in identification and
development of tolerant genotypes. In this study, for
evaluation of salinity effect on expression of HvTIP2;3 and
HvTIP4;1 genes (encoding channel proteins in the
membrane) in the root of barley, three genotypes viz.,
Clipper (salt susceptible), Sahara3771 (salt tolerant) and
advanced breeding line (a salt tolerant line derived from a
cross between Kavir and Sahara genotypes) were planted
under 0, 100 and 200 mMNaCl. Analysis of variance revealed
non-significant differences among genotypes, salinity levels
and sampling stages for HvTIP2;3 and HvTIP4;1 genes
expression pattern, whereas genotype x salinity interaction
for HvTIP2;3 and genotype x sampling stage interaction
were significant for both of the genes studied. The
expression of HvTIP 2;3 gene in the 100 mMNaCl, was
increased in salt susceptible genotype Clipper and
decreased in tolerant genotypes compared with control.
Mean comparison of genotype and sampling stage
combination showed that the expression level of HvTIP4;1
gene 3 weeks after salinity stress was increased in Sahara
and advanced breeding line and decreased in Clipper. The
study revealed that these genes are affected under salinity
stress‚ and their effective utilization may increase salinity
tolerance in plants.

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Published

2017-12-04

How to Cite

Salami, R., Mohammadi, S. A., Ghaffariyan, S., & Moghaddam, M. (2017). Evaluating the expression of Hv TIP2;3 and Hv TIP4;1 in barley genotypes under different levels of salinity stress. The Indian Journal of Genetics and Plant Breeding, 77(4). Retrieved from https://epubs.icar.org.in/index.php/IJGPB/article/view/76266

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