Marker assisted pyramiding of major blast resistance genes Pi9 and Pita in the genetic background of an elite Basmati rice variety, Pusa Basmati 1
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Authors
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Apurva Khanna
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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Vinay Sharma
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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Ranjith K Ellur
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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Asif B. Shikari
Division of Genetics and Plant Breeding, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar 191121, Jammu and Kashmir;
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S. Gopala Krishnan
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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U.D. Singh
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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G. Prakash
Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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T.R. Sharma
ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012
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Rajeev Rathour
Department of Agricultural Biotechnology, Chaudhary Sarwan Kumar Himachal Pradesh Krishi
Vishvavidyalaya, Palampur 176 062, Himachal Pradesh
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Mukund Variar
Central Rainfed Upland Rice Research Station- ICARCRRI, Hazaribagh 825 301, Jharkhand
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S.K. Prashanthi
Agricultural Research Station, Mugad, University of Agricultural Sciences, Dharwad 580 007, Karnataka
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M. Nagarajan
Rice Breeding and Genetics Research Centre, ICAR-IARI, Aduthurai 612 101,Tamil Nadu
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K.K. Vinod
Rice Breeding and Genetics Research Centre, ICAR-IARI, Aduthurai 612 101,Tamil Nadu
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Prolay K. Bhowmick
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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H. Rajashekhara
ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora 263 060, Uttarakhand
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N.K. Singh
ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012
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K.V. Prabhu
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
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Ashok K. Singh
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012
Abstract
Basmati is a premium quality rice of India which is highly
priced in the international market. Pusa Basmati 1, an elite
Basmati rice variety is highly susceptible to rice blast caused
by Magnaporthe oryzae. Therefore, pyramiding blast
resistance genes is essential to effectively combat the blast
disease and increase the durability of resistance genes.
The blast resistance genes Pi9 and Pita have been earlier
demonstrated to be effective in Basmati growing regions
of the country. Therefore, in the present study, monogenicnear isogenic lines Pusa 1637-18-7-6-20 and Pusa 1633-3-8-
8-16-1 carrying Pi9 and Pita, respectively, were intercrossed
to generate pyramided lines through marker assisted
foreground, background and phenotypic selection for
recurrent parent phenotype. The pyramided lines carrying
Pi9+Pita were found to be either at par or superior to the
recurrent parent Pusa Basmati 1 for agro-morphological,
grain and cooking quality traits. Further, these pyramided
lines were also found to show resistance against three
virulent pathotypes of M. oryzae namely, Mo-nwi-kash 1,
Mo-nwi-lon2 and Mo-ei-ran1, when evaluated under artificial
inoculation conditions as well as in the natural epiphytotic
conditions of uniform blast nursery at two locations. The
developed pyramided lines are the potential sources of
blast resistance genes in the Basmati improvement program
and can also be released for commercial cultivation after
required testing.