Assessment of double haploid culture conditions in bell pepper (Capsicum annuum L.)
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Authors
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Anjanabha Bhattacharya
Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
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Bhavesh Palan
Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
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Kishor Mali
Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
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Bharat Char
Mahyco Research Centre, Maharashtra Hybrid Seeds Company Private Limited, Dawalwadi, Jalna, Aurangabad Road, Jalna 431 203, Maharashtra
Abstract
The present study was performed to generate double haploid lines in bell pepper with high regeneration efficiency via anther culture. In total, 825 anthers were plated on five different modified MS media viz., MS I, MS II, MS III, MS IV
and MS V. The number of embryoids formed was found to be highest on MS IV media (26 embryoids) as compared to others. Forty five embryoids were developed on five media combinations, out of which 19 were regenerated into plantlets. Therefore, the optimized media i.e. MS IV in this study may be suitable for the development of double haploid lines in other economically important bell pepper
as well as hot pepper genotypes through anther culture.
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Submitted
2016-11-28
Published
2016-11-29
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Articles
How to Cite
Bhattacharya, A., Palan, B., Mali, K., & Char, B. (2016). Assessment of double haploid culture conditions in bell pepper (Capsicum annuum L.). The Indian Journal of Genetics and Plant Breeding, 75(4). https://epubs.icar.org.in/index.php/IJGPB/article/view/63391