Molecular mapping of rice root-knot nematode (Meloidogyne graminicola) resistance gene in Asian rice (Oryza sativa L.) using STMS markers

Abstract

Rice root-knot nematode, Meloidogyne graminicola is one
of the major pests of rice cropping system and is
responsible for considerable yield reductions. Out of
various management options tried so far, the resistance
against M. graminicola in rice could be the most valuable
in alleviating this problem. In present study a rice cultivar
Abhishek exhibited a strong resistance with least number
of galls (2galls/plant) and a kind of necrotic browning of
roots which is typical of R-gene mediated resistance
response. In order to map the gene governing resistance
to root-knot nematode, the cultivar Abhishek was crossed
with the Bangla Patni, a highly susceptible genotype to
generate F2mapping population. Using bulked segregant
analysis, a marker HvSSR10-21 was identified to be
putatively linked with the resistant locus in cv. Abhishek
with significant LOD score. The significant LOD score value
indicates the linkage between identified marker and the
resistant locus against M. graminicola. We designate this
gene as Mg1(t).