Short Communication : Fertility restorer (Rf) gene linked STMS marker: An efficient tool for testing the genetic purity of hybrid rice (Oryza sativa L.) seed


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Authors

  • Praveen K. Singh Division of Seed Science and Technology, ICAR-Indian Agricultural research Institute, New Delhi 110 012
  • Shiv K. Yadav Division of Seed Science and Technology, ICAR-Indian Agricultural research Institute, New Delhi 110 012
  • Chandra Prakash ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012
  • Sanjay Singh ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012
  • S. K. Chakrabarty Division of Seed Science and Technology, ICAR-Indian Agricultural research Institute, New Delhi 110 012
  • N. K. Singh ICAR-National Research Centre on Plant Biotechnology, New Delhi 110012

Abstract

A fertility restorer (Rf) gene linked STMS marker, RM 6100
as well as Grow Out Test (GOT) were used for testing the
genetic purity of hybrid seed of eight popular rice hybrids.
The genetic purity ranging from 96.00% to 100.00%, could
be determined through marker, whereas, it varied from
97.00% to 99.50% in GOT. The comparative assessment of
both methods exhibited that molecular marker was more
efficient than GOT, as it could detect 0.25-2.50% more
impurities very precisely as compared to GOT.It suggests
that a single Rf gene linked co-dominant STMS marker
appeared to be a simple, precise and quick option over
GOT for testing the purity of hybrid seed lots in short span
of time.

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Submitted

2017-08-25

Published

2017-08-26

Issue

Vol. 77 No. 3 (2017): The Indian Journal of Genetics and Plant Breeding

Section

Articles

How to Cite

Singh, P. K., Yadav, S. K., Prakash, C., Singh, S., Chakrabarty, S. K., & Singh, N. K. (2017). Short Communication : Fertility restorer (Rf) gene linked STMS marker: An efficient tool for testing the genetic purity of hybrid rice (Oryza sativa L.) seed. The Indian Journal of Genetics and Plant Breeding, 77(3). https://epubs.icar.org.in/index.php/IJGPB/article/view/73616