Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii


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Authors

  • Yanan Zhang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
  • Boyun Yang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
  • Liping Luo Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
  • Dongjin Xiong Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
  • Xueyong Huang Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China
  • Huolin Luo Jiangxi Key Laboratory of Plant Resources, School of Life Science, Nanchang University, Nanchang, Jiangxi 330031, People’s Republic of China

Abstract

RT-qPCR is a sensitive, efficient and reliable method for
gene expression studies and reference gene expression
stability was essential for RT-qPCR. Since previous studies
showed that no reference gene could exhibit changeless
expression pattern in all experiments, this study determined
the gene expression stability of 11 reference genes across
various treatments in Anoectochilus roxburghii. The results
indicated that expression stability of genes varied
considerably under different treatments. The genes, GAPDH,
EF1α and ACT1 emerged as the most reliable reference
genes.

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Submitted

2017-08-25

Published

2017-08-26

Issue

Vol. 77 No. 3 (2017): The Indian Journal of Genetics and Plant Breeding

Section

Articles

How to Cite

Zhang, Y., Yang, B., Luo, L., Xiong, D., Huang, X., & Luo, H. (2017). Selection of reliable reference genes for gene expression studies by quantitative real-time PCR in Anoectochilus roxburghii. The Indian Journal of Genetics and Plant Breeding, 77(3). https://epubs.icar.org.in/index.php/IJGPB/article/view/73619