Prevalence of Listeria Monocytogenes in Freshwater Fish of Northeast India and Their Molecular Characterization by PCR


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Authors

  • Raj Kumar Pegu
  • Arnab Sen
  • I. Shakuntala
  • Ashok Kumar
  • Giasuddin Ahmed

Keywords:

Freshwater fish, L. monocytogenes, Antimicrobial resistance, Virulence genes.

Abstract

Five different species of freshwater fish (total number sampled =1,165) were collected from various fish markets of northeast India and screened for the presence of Listeria monocytogenes using enrichment and selective plating methods. Polymerase Chain Reaction (PCR) methods were standardized for confirmation of L. monocytogenes isolates and detection of various virulence genes (hlyA, iap, plcA and plcB). Out of the total samples, 27 (2.31%) L. monocytogenes resistant to three different antibiotics could be isolated with a prevalence rate of 5.45%, 3.67%, 1.43%, 1.38% and 0.33% in C. batrachus, H. fossilis, A. testudinus, M. seenghala, and L. guntea, respectively. Virulent-associated genes; hlyA, iap, plcA and plcB were detected in 40.7%, 29.6%, 40.7% and 22.2% of the 27 isolates, respectively. The prevalence of antibiotic resistant L. monocytogenes harbouring virulence genes in freshwater fish can contaminate other food and food products, thereby affecting human and animals directly or indirectly.

Author Biographies

  • Raj Kumar Pegu
    Department of Biotechnology, Gauhati University, Gopinath Bordoloi Nagar, Guwahati 781014, Assam
  • Arnab Sen
    Division of Animal Health, ICAR Research Complex for NEH Region, Umiam 793103, Meghalaya
  • I. Shakuntala
    Division of Animal Health, ICAR Research Complex for NEH Region, Umiam 793103, Meghalaya
  • Ashok Kumar
    Division of Animal sciences, Indian Council of Agricultural Research (ICAR), Krishi Bhavan, New Delhi 110001
  • Giasuddin Ahmed
    Department of Biotechnology, Gauhati University, Gopinath Bordoloi Nagar, Guwahati 781014, Assam

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Submitted

2018-03-20

Published

2018-03-20

Issue

Vol. 30 No. 2 (2017)

Section

Articles

License

Submission of a manuscript implies that when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher. The Indian Association of Hill Farming (IAHF), as the publisher, has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The IAHF has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to IAHF where information will be utilized.

How to Cite

Pegu, R. K., Sen, A., Shakuntala, I., Kumar, A., & Ahmed, G. (2018). Prevalence of Listeria Monocytogenes in Freshwater Fish of Northeast India and Their Molecular Characterization by PCR. Indian Journal of Hill Farming, 30(2). https://epubs.icar.org.in/index.php/IJHF/article/view/77104