Phytochemical screening of cryo-grinded Ajwain (Trachyspermum ammi L.)
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L.K. Sharma , D. Agarwal , Sapna Jain , S. John , R. Kumar , Amit Bhargav , M.K. Mahatma* & S.N. Saxena
ICAR-National Research Centre on Seed Spices, Ajmer, Rajasthan, India
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Keywords:
Ajwain, amino acid, cryo-grinding, carbohydrate, hexane, methanol, proteinAbstract
Ajwain is a rich source of phytochemicals. To find out best solvent for phytochemical extraction various solvents i.e.
water, alcohol, hydro-alcoholic (70:30), benzene, petroleum ether, hexane and methanol were used. Maximum
extractive value was observed with hexane (16.28) followed by alcohol (16.04) and methanol (14.75) solvent. Further
phytochemicals analysis were carried out using hexan and methanol solvent.Qualitative chemical tests were
conducted to identify various phytoconstituents in all samples. Various tests were performed to identify carbohydrates,
proteins, steroids, amino acids, flavonoids, alkaloids, tennis, vitamin, glycosides and triterpenoids in ajwain. In ajwain
carbohydrate, protein, amino acid, steroids, and flavonoids were detected in both the solvent and all the tests were
positive. Alkaloids, Triterpenoids and vitamin tests were absent in both the solvent of ajwain. Tenins and glycosides
were present in some test of methanol extract and also hexane extract of ajwain. It is considerably easier and cheaper
to perform a preliminary phytochemical screening by using qualitative methods
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