Glutaraldehyde cross linked bubaline acellular periosteum scaffolds augment healing of fractured long bones in dogs
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Authors
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Ghanshyam Patel
Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (UP)-224 229
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Anil Kumar Gangwar
Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (UP)-224 229
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Sangeeta Devikhangembam
Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (UP)-224 229
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Rajesh Kumar Verma
Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya (UP)-224 229
Keywords:
Acellular periosteum, Bone scaffold, Dog, Fracture healingAbstract
In the present study, glutaraldehyde-cross linked bubaline acellular periosteum (GBAP) scaffolds were used to augment long bone fracture healing in dogs. Twelve client-owned male dogs, aged 6-9 yr, with fracture of long bones, were divided randomly into groups A (control) and B (treatment). The fracture was stabilized by intramedullary pinning in both groups. Further, in group B animals, GBAP scaffold was placed circumferentially covering the fractured site. The functional limb usage, callus index, serum calcium, phosphorus, alkaline phosphatase, oxidant and antioxidant status of the patients were evaluated. By day 45, group B animals showed full function at standing and walking, and mineralization of bone at the fracture site. The serum calcium, inorganic phosphorus and alkaline phosphatase levels were higher in treatment group. Levels of MDA, GSH and catalase increased significantly in control group. It was concluded that GBAP scaffolds helped to augment fracture healing and reduce the healing time.
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References
Bae, G.U., Seo, D.W., Kwon, H.K., Lee, H.Y., Hong, S. and Lee, Z.W. 1999. Hydrogen peroxide activates p70sek signaling pathway. J. Biol. Chem. 274: 32596-32602.
Bisla, R.S., Singh, J. and Krishnamurthy, D. 2002. Assessment of oxidative stress in buffaloes suffering from diaphragmatic hernia. Indian J. Vet. Surg. 23: 77-80.
Branden, T.D. and Brinker, W.D. 1973. Effect of certain internal fixation devices on functional limb usages in dogs. J. Am. Vet. Med. Assoc. 162: 642-646.
Chen, Z., Mutoh, M., Sumizawa, T., Furukawa, T., Haraguchi, M. and Tani, A. 1997. Reversal of heavy metal resistance in multi drug resistant human KB carcinoma cells. Biochem. Biophys. Res. Commun. 236: 586-590.
Cohen, G., Dembiec, D. and Marcus, J. 1970. Measurement of catalase activity in tissue extracts. Anal. Biochem. 34: 30-38.
Crapo, P.M., Gilbert, T.W. and Badylak, S.F. 2011. An overview of tissue and whole organ decellularization processes. Biomaterials 32: 3233-3243.
Eastaugh-Waring, S.J., Joslin, C.C., Hardy, J.R.W. and Cunningham, J.L. 2009. Quantification of fracture healing from radiographs using the maximum callus index. Clin. Orthopaed. Rel. Res. 467:1986- 1991.
Fong, K., Truong, V., Foote, C.J., Petrisor, B., Williams, D., Ristevski, B., Sprague, S. and Bhandari, M. 2013. Predictors of nonunion and reoperation in patients with fractures of the tibia: An observational study. BMC Musculoskeletal Disorders 14:103.
Gangwar, A.K., Sharma, A.K., Kumar, N., Kumar, N., Maiti, S.K., Gupta, O.P., Goswami, T.K. and Singh, R. 2006. Acellular dermal graft for repair of abdominal wall defects in rabbits. J. South Afr. Vet. Assoc. 77: 79-85.
Gilbert, T.W., Sellaro, T.L. and Badylak, S.F. 2006. Decellularization of tissues and organs. Biomaterials 27: 3675-3683.
Hegde, Y., Dilipkumar, D. and Usturge, S. 2010. Comparative evaluation of biochemical parameters during fracture healing in dogs. Karnataka J. Agri. Sci. 20: 694-695.
Kruger, T.E., Miller, A.H. and Wang, J. 2013. Collagen scaffolds in bone sialoprotein-mediated bone regeneration. Sci. World J. 23: 22-27.
Masayasu, M. and Hiroshi, Y. 1979. A simplified assay method of superoxide dismutase activity for clinical use. Clin. Chim. Acta. 92: 337-342.
Nimni, M.E., Cheung, D., Strates, B., Kodama, M. and Sheikh, K. 1987. Chemically modified collagen: a natural biomaterial for tissue replacement. J. Biomed. Mater. Res. 21:741-771.
O’Brien, T.K., Gabbay, S., Parkes, A.C., Knight, R.A. and Zalesky, P.J. 1984. Immunological reactivity to a new glutaraldehyde tanned bovine pericardial heart valve. ASAIO J. 30: 440-444.
Oryan, A., Alidadi, S., Moshiri, A. and Maffulli, N. 2014. Bone regenerative medicine: classic options, novel strategies, and future directions. J. Orthopaed. Surg. Res. 9: 1-28.
Placer, Z.A., Cushman, L.L. and Johnson, B.C. 1966. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal. Biochem. 16: 359-364.
Prins, H.K. and Loos, J.A. 1969. Biochemical methods, In: Red Cell Genetics, J.J. Yunis (Ed). Academic Press, New York, USA. pp 115-137.
Reilly, P.M., Schiller, H.J. and Bulkley, G.B. 1991. Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites. Am. J. Surg. 161:488-503.
Saraswathy, G., Sastry, T.P., Pal, S., Sreenu, M. and Kumar, R.S. 2004. A new bio-inorganic composite as bone grafting material: In vivo study. Trends Biomater. Artif. Organs 17: 37-42.
Sontakke, A.N. and Tare, R.S. 2002. A duality in the roles of reactive oxygen species with respect to bone metabolism. Clin. Chim. Acta 318:145-148.
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