Oxidative stress studies in buffaloes undergoing inhalant and total intravenous anaesthesia


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Authors

  • Ram Niwas Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001 (Haryana)
  • R.N. Chaudhary Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001 (Haryana)
  • Dinesh Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001 (Haryana)
  • Rajesh Kumar Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001 (Haryana)

Keywords:

Anaesthesia, Buffalo, Isoflurane, Sevoflurane, Thiopentone, Total Intravenous Anaesthesia

Abstract

The study was conducted on 18 female buffaloes diagnosed with diaphragmatic hernia, which were randomly allocated into three groups (n=6 each). All animals were premedicated with atropine sulphate, xylazine hydrochloride, and butorphanol, followed by anaesthetic induction with thiopentone sodium. Anaesthesia was maintained using a thiopentone continuous rate infusion in the TT group, isoflurane in the TI group, and sevoflurane in the TS group, all delivered via a partial rebreathing system. Enzymatic and non-enzymatic oxidative stress markers, along with antioxidant parameters, were evaluated at six different time points. The results indicated that total intravenous anaesthesia was associated with a lower degree of physiological stress compared to inhalant anaesthesia using either isoflurane or sevoflurane in buffaloes undergoing diaphragmatic herniorrhaphy.

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References

Balaban, R.S., Nemoto, S. and Finkel, T. 2005. Mitochondria, oxidants, and aging. Cell 120: 483-495.

Bedirli, N., Demirtas, C.Y., Akkaya, T. Salman, B., Alper, M., Bedirli, A. and Pasaoglu, H. 2012. Volatile anaesthetic preconditioning attenuated sepsis induced lung inflammation. J. Surg. Res. 178: E17-E23.

Benzie, I.F.F. and Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 239: 70-76.

Cao, G., Alessio, H.M. and Cutler, R.G. 1993. Oxygen radical absorbance capacity assay for antioxidants. Free Radic. Biol. Med. 14: 303-311.

Christopher, G., Evans, L.C. and Jason, E.G. 2010. Heat shock protein 70 (Hsp70) as an emerging drug target. J. Med. Chem. 53: 4585-4602.

Church, D.B., Nicholson, A.I., Ilkiw, J.E. and Emslie, D.R. 1994. Effect of non-adrenal illness, anaesthesia and surgery on plasma cortisol concentrations in dogs. Res. Vet. Sci. 56:129-131.

Desborough, J.P. 2000. The stress response to trauma and surgery. Br. J. Anaesth. 85: 109-117.

Ellis, R.J. and Vandervies, S.M. 1991. Molecular chaperones. Annu. Rev. Biochem. 60: 321-347.

Eunkyoung, K., Zhao, Z., Rzasa, J.R., Glassman, M., Bentley W.E., Chen, S., Kelly, D.L. and Payne, G.F. 2021. Association of acute psychosocial stress with oxidative stress: Evidence from serum analysis. Redox Biol. 47: 1-34.

Fernandez, R.R., Concepcion, Y.M. and Zulueta, G. 2010. 70 kDa heat-shock proteins in surgical stress: thoracotomy vs. herniorrhaphy. Cirugia Espanola 88: 23-29.

Ghiselli, A., Serafini, M., Natella, F. and Scaccini, C. 2000. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radic. Biol. Med. 29: 1106-1114.

Hartl, F.U. and Hayer, H.M. 2002. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 29: 1852-1858.

Janero, D.R. 1990. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Rad. Biol. Med. 9: 515-540.

Kregel, K.C. 2002. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J. Appl. Physiol. 92: 2177-2186.

Lee, J.Y. 2012. Oxidative stress due to anesthesia and surgical trauma and comparison of the effects of propofol and thiopental in dogs. J. Vet. Med. Sci. 74: 663-655.

Lee, Y.M., Song, B.C. and Yeum, K.J. 2015. Impact of volatile anaesthetics on oxidative stress and inflammation. Biomed Res. Int. 242709. doi: 10.1155/2015/242709.

Meistertzheim, A.L, Lejart, M., LeGoic, N. and Thébault, M.T. 2009. Sex-, gametogenesis, and tidal height-related differences in levels of HSP70 and metallothioneins in the Pacific oyster Crassostreagigas. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 152: 234-239.

Morimoto, R.I. and Santoro, M.G. 1998. Stress inducible responses and heat shock proteins: New pharmacologic targets for cytoprotection. Nat. Biotechnol. 16: 833-838.

Saidu, A.M., Bokko, Paul, B., Mohammed, A., Bukbuk, D.N. and Igwenagu, E. 2016. Serum cortisol of Sahel goats following rumenotomy with assorted anaesthetics and sutures. Int. J. Vet. Sci. Med. 4: 23 26.

Santoro, M.G. 2000. Heat shock factors and the control of the stress response. Biochem. Pharmacol. 59: 55 63.

Senoner, T., Schindler, S., Sta ner, S., Ofner, D., Troppmair, J. and Primavesi, F. 2019. Associations of oxidative stress and postoperative outcome in liver surgery with an outlook to future potential therapeutic options. Oxid. Med. Cell. Longev. 1-18.

Su, L.J., Zhang, J.H., Gomez, H., Raghavan, R., Hong, X., Dongxue, X., Jiang, F. and Peng, Z.Y. 2019.Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis. Oxid. Med. Cell. Longev. 1-13.

Tucker, N.R., Ustyugov, A., Bryantsev, A.L., Konkel, M.E. and Shelden, E.A. 2009. Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis. Cell Stress Chaperones. 14: 521-533.

Wang, Z., Ye, Z., Huang, G., Wang, N., Wang, E. and Guo, Q. 2016. Sevoflurane post-conditioning enhanced hippocampal neuron resistance to global cerebral ischemia induced by cardiac arrest in rats through PI3K/Akt survival pathway. Front. Cell.Neurosci.10: 271.doi: 10.3389/fncel.2016. 00271

Submitted

2026-06-12

Published

2026-06-15

Issue

Section

Research Articles

How to Cite

Niwas, R., Chaudhary, R., Dinesh, & Kumar, R. (2026). Oxidative stress studies in buffaloes undergoing inhalant and total intravenous anaesthesia. Indian Journal of Veterinary Surgery, 47(01), 27-30. https://epubs.icar.org.in/index.php/IJVS/article/view/180069