Ozonized autohemotherapy, a novel treatment for teat laceration with fistula in cows: A clinical study

Abstract

The present study evaluated the effect of ozonized autohemotherapy (O3-AHT) on early, scarless teat wound healing in cows. A total of six lactating cows presented with teat wounds associated with fistula formation were included. Following thorough clinical examination, all animals were sedated with inj. xylazine (0.1 mg/kg body weight, i.v.), and a ring block was performed using 2% lignocaine hydrochloride. Patency of the teat canal was maintained by placing a size No. 10 infant feeding tube in situ. Under aseptic conditions, the mucosal and muscular layers were closed using a simple continuous suture pa ern with polyglactin 910 (size 3-0). Subsequently, 2 mL of whole blood collected from each animal was subjected to ozonation and administered subcutaneously at the surgical site. Skin closure was achieved using disposable stainless-steel staples, followed by protection with an adhesive bandage (Dynafix). Postoperatively, all animals received antibiotics, anti-inflammatory therapy, and intramammary treatment. Skin staples were removed on the 7 postoperative day, and wound healing was assessed through morphological and ultrasonographic evaluation. The findings suggest that O3 AHT is a promising complementary therapy to promote early tissue repair and regeneration in bovine teat injuries.