Prediction of Gestational Age and Differential Diagnosis of Pregnancy and Uterine Pathology Using Ultrasonography in Buffalo


Abstract views: 51 / PDF downloads: 61

Authors

  • Jisna Kuttiyeri Sasidharan Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
  • Atul Singh Rajput Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
  • Gangula Athidi Lokavya Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
  • Goutam Kumar Das Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
  • Atul Kumar Singh Tomar Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
  • Manas Kumar Patra Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122

https://doi.org/10.62757/IVA.2024.101.2.49-54

Keywords:

Buffalo, ultrasonography, early pregnancy, amniotic vesicle, embryonic death

Abstract

Ultrasonography is gaining importance as a diagnostic tool in large animal theriogenology in recent years in India. It can be used with a greater accuracy for confirmation of pregnancy and ruling out uterine pathology. The present study described the application of ultrasonography for early pregnancy diagnosis and prediction of gestational age in buffaloes under the organized farm set up. A total of fifty parous Murrah buffaloes at 27 to 53 days gestation were included in the study. The diameter of amniotic vesicles, crown-rump lengths of developing embryo were determined during confirmation of pregnancy using B mode trans-rectal ultrasonography at different days of gestation (days 31 to 53). Attachment of embryo to the uterine wall, development of amniotic vesicle, appearance of limb buds and differentiation of body structures of developing embryo and early fetus were observed. A strong positive correlation observed between the days of gestation and amniotic vesicle diameter (r=0.807, p = 0.001) and days of gestation with crown-rump length (r=0.816, p= 0.001). As incidental findings, two cases of embryonic death based on the presence of empty amniotic vesicle, detachment of chorio-allantoic membranes and changes in amniotic fluid appearance and embryonic degeneration were recorded. Additionally, a case of pyometra and mucometra were also diagnosed during the study. The prediction equation based on the ultrasonographic attributes would be useful in determination of gestational age and confirmation of early pregnancy in buffaloes.

Downloads

Download data is not yet available.

References

Al-Eknah, M.M. and Al-Seaf, A.M. (2014) Ultrasonographic Findings in Early Embryonic Death in Buffalo Cows. Pak. Vet. J. 34(1):134-136.

Ali, A. and Fahmy, S. (2008) Ultrasonographic fetometry and determination of fetal sex in buffaloes (Bubalus bubalis). Anim. Reprod. Sci. 106(1-2):90-99.

Awasthi, M., Abhishek, K., Kavani, F., Siddique, G., Dhami, A. J. (2016) Early pregnancy diagnosis in water buffaloes using transrectal ultrasonography. Indian J. Anim. Reprod. 32:43-46.

Brownlie, T.S., Morton, J.M., and McDougall, S. (2016) Accuracy of fetal age estimates using transrectal ultrasonography for predicting calving dates in dairy cows in seasonally calving herds in New Zealand. N. Z. Vet. J. 64:324–329.

Chaffaux, S., Valon, F., and Martinez, J. (1982) Evolution of the sonographic image of the product of conception of the cow. Bull. Acad. Vet. Fr. 55:213–222.

Dufour, S., Durocher, J., Dubuc, J., Dendukuri, N., Hassan, S., and Buczinski, S. (2017) Bayesian estimation of sensitivity and specificity of a milk pregnancy-associated glycoprotein-based ELISA and of transrectalultrasonographic exam for diagnosis of pregnancy at 28–45 days following breeding in dairy cows. Prev. Vet. Med. 140:122–33.

Dutt, R., Singh, G., Gahalot, S.C., Patil, S.S., Sharma, K., Dhaka, A.P., and Yadav, V. (2019) Mucometra associated with cystic ovarian disease and uterine unicornis in sahiwal cow. Explor. Anim. Med. Res. 8(2):82-83.

Ferreira, J.C.P., Martin, I., Irikura, C.R., Gimenes, L.U., Fujihara, C.J., Jorge, A.M., and Oba, E. (2011) Ultrasonographic monitoring of early pregnancy development in Murrah buffalo heifers (Bubalusbubalis). Livest. Sci. 138(1-3):174-179.

Glatzel, P.S., Ali, A., Gilles, M., and Fidelak, C. (2000) Diagnosis of early pregnancy in 30 water buffalo heifers by trans-rectal palpation with and without ultrasonography. Tierarztl. Umschau, 55(6): 329-332.

Gupta, A. K., Gupta, S.K., Sharma, R.K., and Singh, I. (2016) Ultrasonographic imaging of female reproductive tract disorders in bovines: a review. Vet. World 9(12):1441-1449.

Humble, E., Tripathi, S., and Soodan, J.S. (2016) Ultrasonographic pregnancy diagnosis in buffalo (Bubalus bubalis): current status and future perspectives. Buffalo Bull. 35(3): 251-263.

Ibrahim, M., Dar, S.H., Islam, R., and Firdous, S. (2018) Ultrasonography for reproductive management of buffalo. Int. J. Livest. Res. 8(4):1-11.

Kamboh, A.A., Iqbal, A., and Ahmed, T. (2018) Review on the role of early pregnancy diagnosis in livestock. Vet. World 11(1):104-112.

Karen, A., Darwish, B., and Raman, A.D. (2007) Accuracy of ultrasonography and pregnancy associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 68:1150-1155.

Karen, A.M., Darwish, S., Ramoun, A., Tawfeek, K., Van Hanh, N., De Sousa, N., Sulon, J., Szenci, O, and Beckers, J.F. (2011) Accuracy of transrectal palpation for early pregnancy diagnosis in Egyptian buffaloes. Trop. Anim. Health Prod. 43:5-7.

Kumar, V. and Purohit, G.N. (2009) Ultrasonographic diagnosis of the bovine genital tract disorders. Vet Scan 4(2):733-752.

Lokavya, A., Patra, M., Kohli, K., and Kumar, H. (2020) Diagnosis of Early Fetal Death Using Ultrasonography In Crossbred Cattle: A Report. Indian J. Anim. Reprod. 41(2):66-68.

López-Gatius, F., and García-Ispierto, I. (2010) Ultrasound and Endocrine Findings that Help to Assess the Risk of Late Embryo/Early Foetal Loss by Non-Infectious Cause in Dairy Cattle. Repro. Domest. Anim. 45:15–24

Patra, M.K., Rajput, A.S., Sasidharan, J.K., Raza, R.A., Puttanarsappa, T.M., Lokavya, A., Tomar, A.K.S., Das, G.K., Ghosh, S.K., and Gaur, G.K. (2023) Ultrasonography Assisted Estrus Detection Improves the Submission Rate in Prostaglandin Treated Sub-Estrus Murrah Buffaloes. Indian J. Anim. Reprod. 44(1):45–49. 10.48165/ijar.2023.44.01.9

Pieterse, M.C., O Szenci, A.H., Willemse, C.S.A., Bajcsy, S.J., Dieleman, and M.A.M Taverne (1990) Early pregnancy diagnosis in cattle by means of linear array real-time ultrasound scanning of uterus and a qualitative and quantitative milk progesterone test. Theriogenology 33: 697-707.

Sasidharan, J.K., Patra, M.K., Khan, J.A., Singh, A.K., Karikalan, M, De, U.K., Saxena, A.C., Dubal, Z.B., Singh, S.K., Kumar, H., and Krishnaswamy, N. (2023) Differential expression of inflammatory cytokines, prostaglandin synthases and secretory leukocyte protease inhibitor in the endometrium and circulation in different graded CEH-pyometra in bitch. Theriogenology 197:139-149.

Submitted

2024-02-23

Published

2024-02-23

How to Cite

Jisna Kuttiyeri Sasidharan, Atul Singh Rajput, Gangula Athidi Lokavya, Goutam Kumar Das, Atul Kumar Singh Tomar, & Manas Kumar Patra. (2024). Prediction of Gestational Age and Differential Diagnosis of Pregnancy and Uterine Pathology Using Ultrasonography in Buffalo. The Indian Veterinary Journal, 101(2), 49-54. https://doi.org/10.62757/IVA.2024.101.2.49-54