Development of PCR-based DNA markers for identification of hybrids of Tor putitora and Tor khudree
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Authors
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Rutuparnna Priyadarshini
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Annam Pavan Kumar
CIFE
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Vivek Viswas Rao
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Dhalongsaih Reang
ICAR-Central Institute of Fisheries Education
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Gireesh Babu Pathakota
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Aparna Chaudhari
Keywords:
Internal Transcribed Spacer Sequence, 18S rRNA, Mahseer, DNA MarkersAbstract
The genetic identification and characterization of Tor putitota, Tor khudree and their hybrids hold profound
significance in both fundamental biological exploration and practical domains like conservation biology and
fisheries management. This study endeavors to establish a panel of PCR-based DNA markers capable of
accurately discerning between T. putitora, T. khudree, and their hybrid counterparts. Controlled conditions
were employed to generate hybrids of T. putitora and T. khudree, serving to validate the developed markers.
For hybrid identification, primers were devised targeting the nuclear DNA region of ITS1-5.8S-ITS2-28S rRNA.
Post-optimization, the primers MHITSF and MH28SR yielded 700 bp and 500 bp amplicons for pure Tor
khudree and Tor putitora, respectively. Remarkably, Tor putitora X Tor khudree hybrids displayed both 700 bp
and 500 bp amplicons. These markers present a valuable tool for screening mahseer hybrids, thereby
enhancing their management and conservation prospects.
