Identification of a pubescence locus in Chinese flowering cabbage (Brassica rapa L.)
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Authors
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Tetsuya Kitanishi
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan
Author
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Gento Tsuji
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan
Author
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Nakao Kubo
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Sakyo-ku, Kyoto 606-8522, Japan
Author
Keywords:
Brassica rapa,, CAPS marker, Chinese flowering cabbage, GL1, pubescenceAbstract
The pubescence is an important trait for Brassica rapa vegetables concerning insect resistance but causes a bad mouthfeel
when eaten. Pubescence is observed in some Chinese flowering cabbage (B. rapa) cultivars. The cleaved amplified
polymorphic sequence (CAPS) markers linked to pubescence-related genes BrTTG1 and BrGL1 were investigated in a
segregating population. The correlation between the presence/absence of pubescence and DNA marker genotypes tested
using the Mann-Whitney U test, showing that the pubescence trait was significantly correlated with the CAPS marker
linked to BrGL1. Sequence comparison of BrGL1 showed several nucleotide polymorphisms between parental cultivars, of
which 2-bp deletion caused a frame-shift mutation in the BrGL1 of hairless cultivar ‘Hanakazari’. A CAPS marker developed
from BrGL1 sequences co-segregated with the pubescence trait in this population. Therefore, it suggested that the BrGL1
is a likely candidate gene controlling the pubescence trait in this Chinese flowering cabbage line.
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