Transcriptomic dissection of cross kingdom crosstalk in Brassica juncea- Sclerotinia sclerotiorum pathosystem
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Authors
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Kusum Rana
Department of Biotechnology, Punjab University, Chandigarh-160 014, India
Author
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Simarjeet Kaur Sra
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 004, India
Author
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Chhaya Atri
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 004, India
Author
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Seema Rana
Department of Entomology, Texas A&M University, College Station, TX-77843, US
Author
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Jagtar Singh
Department of Biotechnology, Punjab University, Chandigarh-160 014, India
Author
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Surinder S Banga
Department of Entomology, Texas A&M University, College Station, TX-77843, US
Author
Keywords:
Brassica juncea, differentially expressed genes, Sclerotinia sclerotiorum, resistance, RNA-sequencingAbstract
Sclerotinia sclerotiorum is a highly destructive phytopathogen with a broad host range. It causes severe productivity
losses in food and vegetable crops worldwide. Understanding host-pathogen interactions is essential for developing
effective control strategies. In this study, we investigated transcriptomic changes in stem lesions caused by the virulent
isolate PAU (SsLd1-White), of S. sclerotiorum on resistant and susceptible pools derived from Brassica juncea advanced
generation introgression lines, 24 hours post-inoculation (hpi), alongside controls. Gene Ontology enrichment and
KEGG pathway analyses helped to characterize transcriptional dynamics between the two pools. Comparative transcriptome
analysis revealed 3,627 and 10,524 differentially expressed genes (DEGs) uniquely expressed in the resistant and
susceptible pools, respectively, along with 1,807 DEGs commonly expressed in both pools at 24hpi. Several DEGs were
associated with key physiological and metabolic processes, including response to wounding, systemic acquired resistance,
oxidative and osmotic stress, fungal defense, signal transduction, and jasmonic acid signaling. The study provides
novel insights into the molecular basis of the B. juncea-S. Sclerotiorum pathosystem, contributing to a deeper
understanding of host-pathogen interactions and informing future resistance breeding and management strategies.
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