Genetic diversity in castor (Ricinus communis L.) pistillate lines using EST-SSR markers

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  • B USHA KIRAN ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • C LAVANYA ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana


Castor, EST-SSRs, Genotypes, Pistillate lines


Genetic diversity is essential for crop genetic improvement. Genetic diversity among breeding lines helps breeders in selecting parents for hybrid production with maximumheterosis and combining useful genes in a genetic background. However, heterosis levels in castor hybrids are not up to the desired extent due to narrow range of diversity between male and female lines and development of parents from few common sources. The objective of the study was to assess the diversity and genetic relationship of castor pistillate lines using EST-SSR markers. Sixty percent of the SSR used were polymorphic and 59 alleles were observed with an average of 2.4 alleles per locus. Dice similarity coefficient of UPGMA cluster analysis was used to construct dendrogramand observed five clusters. The Dice coefficient ranged from 0.48 to 0.84. Low level of genetic variation was observed in castor at DNA level with SSR markers.


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Allan G, Williams A, Rabinowicz P D, Chan A P, Ravel J and KeimP 2008. Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLP and SSRs. Genetic Resources and Crop Evolution, 55: 365-378.

Anjani K, Yadav W S and Duhoon S S 1999. Collecting castor (Ricinus communisL.) germplasmin north western India. Plant Genetic Resources Newsletter, 120: 48-51.

Ankineedu G and Ganga Prasada Rao N 1973. Development of pistillate castor. Indian Journal of Genetics and Plant Breeding, 33: 416-422.

Bajay M M, Pinheiro J D, Batista C E A, Nobrega M B M and Zucchi M I 2009. Development and characterization of microsatellite markers for castor (Ricinus communis L.), an important oleaginous species for biodiesel production. Conservation of Genetic Resources, 1(1): 237-239.

Bhaavesh B G, Nitish Kumar, Amritpal S S, Bhupendra S P, Ravikiran R, Subhash N and Jadeja G C 2010. Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers. Industrial Crops and Products, 32: 491-498.

Doyle J J and Doyle J L 1987. A rapid DNA isolation procedure for small quantities offresh leaftissue. PhytochemicalBulletin, 19: 11-15.

Foster J F, Allan G J, Chan A P, Pablo D, Rabinowicz, Jacques Rave, Paul J Jackson and Paul Keim 2010. Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis). BMC Plant Biology, 10: 13.

Govaerts R, Frodin D G and Radcliffe-Smith A 2000. World checklist and bibliography ofEuphorbiaceae (with Pandaceae). Redwood Books Limited, Trowbridge, Wiltshire.

Kanti M, Ramya K T, Anjani K and Usha kiran B 2015. Agro-morphological and molecular diversity in castor (Ricinus communisL) germplasmcollection fromAndaman and Nicobar islands, India. Czech Journal of Genetics and Breeding, 12:4-10.

Kavani R H, Vachhani J H, Madariya R B, Jadeja S R and Dobariya K L 2016. Combining ability for seed yield and its components in castor (Ricinus communis L.). Journal of Oilseeds Research, 33(1): 22-25.

Lavanya C 2002. Sensitivity and variations of sex expression in response to environmental changes in castor. Indian Journal of Genetics and Plant Breeding, 62: 232-237.

Lavanya C and Gopinath V 2008. Study on inheritance of morphological characters and sex expression in pistillate lines of castor. Indian Journal of Genetics and Plant Breeding, 68: 275-282.

Lavanya C and Solanki S S 2010. Crop improvement of castor: the challenges ahead. In: Research and Development in Castor: Present Status and Future Strategies, Hegde (Ed.), Indian Society of Oilseeds Research, Hyderabad, pp. 36-55.

Lavanya C 2009. Diversification of the pistillate base using geographically diverse castor germplasm. Proceedings of National Symposium on "Recent Global Developments in the Management of Plant Generic Resources", 17-18th December 2009, NBPGR, New Delhi.

Nybom H 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13: 1143-1155.

Pranavi B, Sitaram G, Yamini K N and Dinesh kumar V 2011. Development of EST-SSR markers in castor bean (Ricinus communis L.) and their utilization for genetic purity testing of hybrids. Genome, 54 (8): 685-691.

Qiu L, Yang C, Tian B, Yang J B and Liu A 2009. Exploiting EST database for the development and characterization ofEST-SSR markers in castor bean (Ricinus communis L). BMC Plant Biology, 10: 278-287.

Rajiv Kumar, Patel J A, Patidar D, Patel N, Patel S, Sapre S and Ravi Shah 2015. Effect of ethrel spray on ISF induction in pistillate line of castor SKP-84. Journal of Oilseeds Research, 32(1): 100-101.

Rao C H, Lavanya C, Anjani K, Sujatha M, Lakshmamma P and Mukta N 2003. Crop improvement in castor. In: Castor in India, Hegde, D.M., Sujatha, M., Singh, N.B. (Eds.). Directorate of Oilseeds Research, Hyderabad, pp. 14-35.

Rohlf F J 1992. NTSYS-PC: Numerical Taxonomy and Multivariate Analysis System Version 2.02k. State University of New York, Stony Brook, New York.

Sunil N, Ashok Kumar A, Ashokvardhan Reddy P and Varaprasad K S 2005. Collection of diversity in castor (Ricinus communis L.) germplasm from parts of Andhra Pradesh. Indian Journal of Plant Genetic Resources, 18: 187-190.

Tessier C, David J, This P, Boursiquot J M and Charrier A 1999. Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theoretical and Applied Genetics, 98: 171-177.

Vivodik M, Balazova Z and Galova Z 2014. RAPD analysis of genetic diversity of castor bean. International Journal of Biological, Veterinary, Agricultural and Food Engineering, 98: 171-177.

Wang H Y, Wei Y M, Yan Y H and Zheng Y L 2007. EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections. Genetics, 48: 35-42.

Williams J G K, Kubelik A R, Livak K J, Rafalski J A and Tingey S V 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18:6531-6535.






How to Cite

B USHA KIRAN, & C LAVANYA. (2016). Genetic diversity in castor (Ricinus communis L.) pistillate lines using EST-SSR markers: GENETIC DIVERSITY IN CASTOR PISTILLATE LINES USING EST-SSR MARKERS. Journal of Oilseeds Research, 33(3).