Development of 2A-CHYSEL technology based multicistronic expression systems for imparting fungal tolerance
Development of 2A-CHYSEL technology based multicistronic expression systems for imparting fungal tolerance
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Authors
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KONDA ARAVIND KUMAR
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad- 500 030
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P B KIRTI
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad- 500 030
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V DINESH KUMAR
ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030
Keywords:
CHYSEL technology, Multigene construct, Necrotrophic fungal resistanceAbstract
Multigene transfer is an approach for development of transgenic crops simultaneously expressing many genes. With an aim to impart necrotrophic fungal tolerance through transgenic approach, the present study describes the development of multigene construct bearing three antifungal genes as a single transcriptional unit capable of expressing at high levels and evading transgene silencing.
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References
Pablo de Felipe 2004. Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genetic Vaccines and Therapy, 2:13-18. DOI: https://doi.org/10.1186/1479-0556-2-13
Konda A K, Narasimha R, Kompelli S K, and Dinesh Kumar V 2009. Codon optimization and PCR based synthesis of an antifungal plant defensin RsAFP2 for expressing in Castor. Journal of Oilseeds Research, 26:210-213.
Konda A K, Narasimha R, Kompelli S K, and Dinesh Kumar V 2010. Modification of ThEn42gene for improved tolerance against Botrytis in castor. Journal of Oilseeds Research, 27:15-17.
