Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame


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Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame

Authors

  • MAINI BHATTACHARJEE Dept. of Genetics and Plant Breeding, Institute of Agril. Science, University of Calcutta, 51/2 Hazra Road Kolkata-700 019, West Bengal
  • TAPASH DASGUPTA Dept. of Genetics and Plant Breeding, Institute of Agril. Science, University of Calcutta, 51/2 Hazra Road Kolkata-700 019, West Bengal

https://doi.org/10.56739/jor.v37iSpecialissue.139122

Keywords:

SCoT markers, EST-SSR markers, PIC value, Sesame, Diversity

Abstract

Expressed sequence tag (EST)-SSR markers and Start codon targeted (SCoT) markers were compared to estimate genetic diversity among thirty sesame genotypes. To achieve this goal, the available sesame database was used as a resource for the development of novel microsatellite (SSR) and Start codon targeted (SCoT) markers. EST- SSR markers detected a mean of 4.34 alleles with a mean polymorphic information content (PIC) of 0.84, against SCoT markers with a mean of 9.6 alleles and a mean PIC of 0.79. SCoT markers amplified more null alleles than EST-SSRs. Geographical diversity did not corroborate with genotypic diversity as the cluster composition revealed that genotypes belonging to different geographical origin grouped in the same cluster.

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References

Bhattacharjee M, Prakash S H, Roy S, Begum T and Dasgupta T 2018. DNA fingerprinting of CUMS 17 (Suprava): a newly developed variety of sesame. International Journal of Pure Applied Biosciences, 6(5):161-166.

Bhattacharjee M, Prakash SH, Roy S, Soumen S, Begum T and Dasgupta T 2019. SSR based DNA fingerprinting of 18 elite Indian varieties of sesame (Sesamum indicum L.). Nucleus https://doi.org/10.1007/s13237-019-00290-3.

Collard B C and Mackill D J 2009. Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27(1): 86.

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Submitted

2023-07-11

Published

2020-03-12

Issue

Vol. 37 No. Specialissue (2020): Journal of Oilseeds Research

Section

Articles

License

The Journal of Oilseeds Research reserves all the copy rights of the published articles and prior permission is to be taken from the journal office if any part of the published articles is to be reproduced in any form in any other publication.

How to Cite

MAINI BHATTACHARJEE, & TAPASH DASGUPTA. (2020). Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame: Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame. Journal of Oilseeds Research, 37(Specialissue). https://doi.org/10.56739/jor.v37iSpecialissue.139122