Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame
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Comparison of Start Codon Targeted (SCoT) and EST-SSR markers in sesame
Keywords:
SCoT markers, EST-SSR markers, PIC value, Sesame, DiversityAbstract
Expressed sequence tag (EST)-SSR markers and Start codon targeted (SCoT) markers were compared to estimate genetic diversity among thirty sesame genotypes. To achieve this goal, the available sesame database was used as a resource for the development of novel microsatellite (SSR) and Start codon targeted (SCoT) markers. EST- SSR markers detected a mean of 4.34 alleles with a mean polymorphic information content (PIC) of 0.84, against SCoT markers with a mean of 9.6 alleles and a mean PIC of 0.79. SCoT markers amplified more null alleles than EST-SSRs. Geographical diversity did not corroborate with genotypic diversity as the cluster composition revealed that genotypes belonging to different geographical origin grouped in the same cluster.
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References
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