In vitro regeneration of castor (Ricinus communis L.)
In vitro regeneration of castor (Ricinus communis L.)
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Keywords:
Castor, tissue culture, TDZ, BAP, shoot induction, callus, rootingAbstract
Standardization of a reliable regeneration protocol is important in castor to develop transgenics conferring resistance to biotic and abiotic stresses. An attempt has been made to developan in vitro regeneration protocol using the hypocotyl explants derived from seedlings obtained from decoated seeds. Out of 60 different hormonal combinations tried, three combinations showed better shoot induction and the rooting was better with IBA 1.0 mg/L.
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References
Alam I, Shamima A S, Sanjoy C M, Md. Jahangir A, Muhammad Khalekuzzaman, A M and Alam M F 2010. In vitro micropropagation through cotyledonary node culture of castor bean (Ricinus communis L.). Australian Journal of Crop Science, 4(2):81-84.
Alejandro G C et al., 2019. In vitro regeneration of castor bean plants (Ricinus communis L.) using epicotyls. Bioscience Journal, 35 (2): 347-355.