Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern and Coss.)


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Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern and Coss.)

Authors

  • H D PUSHPA ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • D K YADAVA ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • SUJATA VASUDEV ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • V VINU ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • CHANDANABEHERA ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • NAVEEN SINGH ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana

https://doi.org/10.56739/jor.v37iSpecialissue.139386

Keywords:

Glucosinolates, Molecular markers, Mustard, SSR markers

Abstract

The study was carried out to identify molecular markers linked to low glucosinolates in Brassica juncea using recombinant inbred lines developed from cross between Pusa Mustard 21 × EC597325. Parental polymorphism studies with 594 SSR markers, revealed 62 informative markers. The bulk segregant analysis (BSA) revealed four microsatellite markers differentiating both the parental lines and the high and low glucosinolates bulks, hence putatively linked to the genomic regions controlling low glucosinolates.

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References

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Griffiths D W, Birch A N E and Hillman J R 1998. Antinutritional compounds in the Brassicaceae: Analysis, biosynthesis, chemistry and dietary effects. Journal of Horticultural Science and Biotechnology, 73: 1–18.

Kumar S, Yadav S K, Chauhan I S, Singh A K, Khan N A and Kumar P R 2004. Total glucosinolate estimation by complex formation between glucosinolate and tetra- chloropalladate (II) using ELISA reader. J. Food Sci. Tech. 41: 63 – 65.

Michelmore R W, Paran I and Kesseli R V 1991. Identification of markers linked to disease-resistance genes by bulked segregant anaylsis: A rapid method to detect markers in specific genomics regions by using segregating populations. Proc. Nat. Acad .Sci. USA. 88: 9828-9852.

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Submitted

2023-07-18

Published

2020-03-12

How to Cite

H D PUSHPA, D K YADAVA, SUJATA VASUDEV, V VINU, CHANDANABEHERA, & NAVEEN SINGH. (2020). Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern and Coss.): Identification of new molecular markers for low glucosinolates in Indian mustard (Brassica juncea L. Czern and Coss.). Journal of Oilseeds Research, 37(Specialissue). https://doi.org/10.56739/jor.v37iSpecialissue.139386