Assessment of genetic purity of two sunflower (Helianthus annuus L.) hybrids using sequence characterized amplified region (SCAR) markers


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Assessment of genetic purity of two sunflower (Helianthus annuus L.) hybrids using sequence characterized amplified region (SCAR) markers

Authors

  • S ANUSHA ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • W DHAMMAPRAKASH ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • K N YAMINI ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • C H ANIL KUMAR ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana
  • V DINESH KUMAR ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad-500 030, Telangana

https://doi.org/10.56739/jor.v37iSpecialissue.139465

Keywords:

Genetic purity, Hybrid PCR, RAPD, SCAR, Sunflower

Abstract

Random amplified polymorphic DNA (RAPD) technique is easier to identify true hybrids. However, it has inherent disadvantage of less reproducibility and that can be overcome by conversion of RAPD markers into sequence characterized amplified region (SCAR) markers. In the present investigation, RAPD analysis was carried out with the two hybrids, KBSH 41 and KBSH 42 along with their parents, polymorphic markers were identified and those markers were converted into SCAR markers. These markers would be of immense potential during routine genetic purity assessment of the two sunflower hybrids.

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References

Doyle J J and Doyle J L 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19:11-15.

Paran I and Michelmore R 1993. Development of reliable PCRbased markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics, 85: 985-993.

Wankhede D 2004. Assessment of genetic purity and diversity among hybrids and varieties of sunflower using morphological and molecular markers. M.Sc. Thesis. ANGRAU, Hyderabad.

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Submitted

2023-07-19

Published

2020-03-12

How to Cite

S ANUSHA, W DHAMMAPRAKASH, K N YAMINI, C H ANIL KUMAR, & V DINESH KUMAR. (2020). Assessment of genetic purity of two sunflower (Helianthus annuus L.) hybrids using sequence characterized amplified region (SCAR) markers: Assessment of genetic purity of two sunflower (Helianthus annuus L.) hybrids using sequence characterized amplified region (SCAR) markers. Journal of Oilseeds Research, 37(Specialissue). https://doi.org/10.56739/jor.v37iSpecialissue.139465