Standardization of seed viability testing through tetrazolium in oil palm (Elaeis guineensis L.)


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Standardization of seed viability testing through tetrazolium in oil palm (Elaeis guineensis L.)

Authors

  • G RAVICHANDRAN ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, Andhra Pradesh
  • B KALYANA BABU ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, Andhra Pradesh
  • P ANITHA ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, Andhra Pradesh
  • G SOMASUNDARAM ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, Andhra Pradesh
  • H P BHAGYA ICAR-Indian Institute of Oil Palm Research, Pedavegi-534 475, Andhra Pradesh

https://doi.org/10.56739/jor.v37iSpecialissue.140940

Keywords:

Oil palm, Seed viability, Tetrazolium

Abstract

To evaluate the seed viability in the zygotic embryo of oil palm through quick test, different tetrazolium (TZ) concentrations, staining temperatures, staining timings and staining pattern were evaluated in the Dura x Pisifera (D x P) crossed seeds. The best staining was obtained with 1 % TZ solution under 30oC for 5 hours kept under complete dark. The staining pattern on the embryo indicated that the top portion towards the operculum had given proper growth in vitro. Hence, TZ staining was found suitable indicator for seed viability in oil palm seed.

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References

Eddy S Kelanaputra, Stephen PC, Nelson, Umi Setawati, Baihaqi Sitepu, Fazrin Nur, Brian P Forster and Abdul R Purba 2018. Seed Production in Oil palm: A Manual. CABI Publication, UK.

Grout BWW, Shelton K and Pritchard H W 1983. Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation. Annals of Botany, 52(3): 381-84.

Lamarca, Edmir Vicente and Barbedo C Jose 2014. Methodology of the tetrazolium test for assessing the viability of seeds of Eugenia brasliensis Lam, Eugenia uniflora L and Eugenia pyriformiscambess. Journal of Seed Science, 36(4): 427-434.

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Submitted

2023-08-16

Published

2020-03-12

How to Cite

G RAVICHANDRAN, B KALYANA BABU, P ANITHA, G SOMASUNDARAM, & H P BHAGYA. (2020). Standardization of seed viability testing through tetrazolium in oil palm (Elaeis guineensis L.): Standardization of seed viability testing through tetrazolium in oil palm (Elaeis guineensis L.). Journal of Oilseeds Research, 37(Specialissue). https://doi.org/10.56739/jor.v37iSpecialissue.140940