SUCROSE SYNTHESIS AND ACCUMULATION IN CONTRASTING COMMERCIAL HYBRIDS OF SUGARCANE


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Authors

  • S Vasantha ICAR-Sugarcane Breeding Institute
  • S Venkatramana ICAR-Sugarcane Breeding Institute
  • Raja Arun kumar ICAR-Sugarcane Breeding Institute

Abstract

Sugarcane has been bred with a focus of obtaining high sugar varieties. Identifying associated traits assumes importance for genetic manipulation. Enzymes of sucrose metabolism were researched and reviewed over decades. In the present study sucrose content in individual internodes, and related enzymes viz., invertases (acid and neutral) and sucrose phosphate synthetase (SPS) were assessed. Sucrose content ranged from 119 mg/g to 432 mg/g, while acid invertase (AI) ranged from 1.35 μ mol g-1 h-1 to 52.3 μ mol g-1 h-1 Enzymes SPS did not show any specific trend among different internodes; however the SPS/AI ratio showed significant correlation with sucrose content. Significant differences were observed between high and low sugar varieties with regard to the parameters studied.

Author Biographies

  • S Vasantha, ICAR-Sugarcane Breeding Institute
    Principal Scientist (Plant Physiology)
  • S Venkatramana, ICAR-Sugarcane Breeding Institute
    Principal Scientist (Plant Physiology) [Retd.]
  • Raja Arun kumar, ICAR-Sugarcane Breeding Institute
    Scientist (Plant Physiology)

References

Ashwell ,G (1957) .In : method in enzymol 3 (eds Colowick, S.J and Kaplan, N.O)Academic Press New york: p-75.

Beruter, J and Studer ,F.M.E(1997). The effect of girdling on carbohydrate partitioning in the growing apple fruit. J. plant physical.,151: 277 – 285.

Botha, F.C and Black, K.G (2000) Sucrose phosphate synthase and sucrose synthase activity during the maturation of internodal tissue in sugarcane.Australian Journal of plant physiology,27(1) : 81 – 85.

Botha F C, Whittaker A,Voster DJ,BlackK G (1996). Sucrose accumulation rate , carbon partitioning and expression of key enzyme activities in sugarcane stem tissue. In sugarcane research towards efficient and sustainable production. Wilson , J.R, Hogarth , D.M, Campbell , J.A. and Garside , A.L. (eds), CSIRO Division of Tropical crops & Pastures, Brisbane, 16 : Pp.98 – 101.

Christopher PLGrof,Deon P Knight, Scott D McNeil, John E Lunn and James A (1998). A modified assay method shows leaf sucrose-phosphate synthase activity is correlated with leaf sucrose content across a range of sugarcane varieties. Australian Journal of Plant Physiology 25(4) 499 - 502

Grof C A and JA Campbell (2001). Sugarcane sucrose metabolism :scope for molecular manipulation. Australian. j .plant physiol.,28 : 1-12.

Hatch, M.D and Glasziou.(1963). Sugar accumulation cycle in sugarcane ll. Relationship of invertase Activity to sugar content and growth rate in storage tissue of plants grown in Controlled environments. Plant Physicol ,38 :338 – 343.

Hubbard ,N. L..Huber,S.C and pharr,D.M (1989).Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon(Cucumismela l.)fruits. Plant physiology, 91 : 1527-1534.

Huber, S. C. and Huber, J. L (1996). Role and regulation of sucrose-phosphate synthase in higher plants.Annu. Rev. Plant Physiol. Mol. Biol. 47, 431±444

Jackson, P. Bonnett G. chudleigh P. Hogarth M. Wood A (2000).The Relative importance of caneyield& tracts affecting CCS in sugarcane Varities in Hogarth D.M.ed. Proc. Aust .Soc . Sugarcane Tech nol – 22 ;22 –29.

Lingle, S.E (1999). Sugarcane metabolism during growth & development in sugarcane internodes.Crop science ,39; 480 – 486.

Madan, V.K. Singh,K.Pande , H.P and Saxena , Y.R (1981). Foliar enzymes of sugarcane seasonal varieties of invertases. Int sugar j ,83 : 163.

Moore, P.H (1995). Temporal and spatial regulation of sucrose accumulation In the sugarcane stem. Aust .J. Plant Physiol .22 ;661 – 679.

Schaffer, A.A (1986). Invertases in young mature leaves of Citras Sinensis Phytochemistry.25; 2275 – 2277.

Stitt, M., Huber, S., and Kerr, P(1987). Control of photosynthetic sucrose formation. In The Biochemistry of Plants, Vol. 10: Photosynthesis, M.D. Hatch and N.K. Boardman, eds (New York: Academic Press), pp. 327-409.

Stitt ,M. Ingo Wilke , Regina feil and Hans W.Heldt (1988). Coarse control of sucrose –phosphate synthase in leaves : Alterations of the kinetic properties in response to the rate of photosynthesis and the accumulation of sucrose. Planta, 174 : 217-230.

Stitt, M., and Quick, P (1989). Photosynthetic carbon partitioning: Its regulation an possibilities for manipulation. Physiol. Plant. 77, 633-641.

Venkataramana, S. Naidu ,KM and Singh (1991). Invertases and growth factors dependent sucrose accumulation in sugarcane. Plantscience , 74 : 65-72.

Venkataramana S and Naidu KM (1993). Invertase – sucrose relationship in young & mature stem of sugarcane Phytochemistry , Vol. 32,No: 4 :PP.82 - 822.

Vorster, D.J. and Botha ,F.C (1998). Partial purification & characterization of sugarcane neutral invertase. Phytochemistry . 49; 651 – 655.

Zhu Y.J. Komor E. Moore PH (1997). sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol. 115; 609 – 616.

Zhu., J., Albert , H.H and Moore, P.H (2000). Differential expression of soluble acid invertase genes in the shoots of high – sucrose & low – sucrose species of Saccharum & their hybrids. Aust J. Plant Physicol.27: 193 – 199.

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Submitted

18-03-2019

Published

02-10-2019

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Section

Research Article

How to Cite

Vasantha, S., Venkatramana, S., & Arun kumar, R. (2019). SUCROSE SYNTHESIS AND ACCUMULATION IN CONTRASTING COMMERCIAL HYBRIDS OF SUGARCANE. Journal of Sugarcane Research, 9(1). https://epubs.icar.org.in/index.php/JSR/article/view/87968