CLONING AND CHARACTERIZING THE FOLMER REGION OF MTCOI GENE DIAGNOSTIC FOR SUGARCANE TOP BORER, SCIRPOPHAGA EXCERPTALIS (WALKER) (LEPIDOPTERA: PYRALIDAE)
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Authors
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T Ramasubramanian
ICAR-Sugarcane Breeding Institute, Coimbatore - 641 007
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R.K Nilavuckkarasi
ICAR-Sugarcane Breeding Institute, Coimbatore - 641007
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C Yogambal
ICAR-Sugarcane Breeding Institute, Coimbatore - 641007
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K Ramaraju
Tamil Nadu Agricultural University, Coimbatore - 641 003
Abstract
Folmer region of mtCOI gene from sugarcane top borer, Scirpophaga excerptalis (Walker) (Lepidoptera: Pyralidae) has been cloned and characterized. This would be the ideal DNA barcode as it is 658 bp in size (KJ013411) and does not have any stop codon in its amino acid sequence. The COI fragment cloned in this study showed less than 89% identity with other species of Scirpophaga having full length DNA barcodes and hence, it clearly delineates S. excerptalis from other closely related congenerics. The COI gene sequence of S. excerptalis would also serve as a base for identifying the cryptic species if any, in the sugarcane crop ecosystem of the Indian subcontinent.References
Asokan R, Rebijit KB, Srikumar KK, Shivarama Bhat P, Ramamurthy VV (2012) Molecular identification and diversity of Helopeltis antonii and Helopeltis theivora (Hemiptera: Miridae) in India. Florida Entomologist 95(2): 350-358.
Banerji SN, Butani DK (1975) Insect pests of sugarcane and their control. Indian Sugar 25: 414-416 and 561-571.
Box HE (1953) List of sugarcane insects. Commonwealth Institute of Entomology, London 101p.
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3(5): 294-299.
Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences 270: 313-321.
Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W (2004) Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the USA 101(41): 14812-14817.
Jalali SK, Ojha R, Venkatesan T (2015) DNA Barcoding for Identification of Agriculturally Important Insects. In: Chakravarthy AK (ed.) New Horizons in Insect Science: Towards Sustainable Pest Management, Springer India pp. 13-22.
Lewanich A (1981) A review of the old world species of Scirpophaga (Lepidoptera: Pyralidae). Bulletin of the British Museum (Natural History) 42:185-298.
Mukunthan N (1986) The Top Borer, Scirpophaga excerptalis Walker. In: David H, Easwaramoorthy S, Jayanthi R (eds.) Sugarcane Entomology in India, Sugarcane Breeding Institute, Coimbatore pp. 93-119.
Ojha R, Jalali SK, Mushtak Ali TM, Venkatesan T, Prosser SW, Krishna Kumar NK (2014) DNA barcoding of Indian ant species based on cox I gene. Indian Journal of Biotechnology 13:165-171.
Ramasubramanian T, Ramaraju K (2014a) DNA barcode of sugarcane root aphid, Tetraneura javensis (van der Goot). Research paper presented in the National Symposium on Phenomenal Science-BioGalaxia-2014 held on 17 October 2014 at Bharathiar University, Coimbatore, Abstract book p. 14.
Ramasubramanian T, Ramaraju K, Pandey SK (2014b) DNA barcode of Sesamia inferens (Walker). Research paper presented in the National Symposium on Bioenergy for Sustainable Development-The Potential Role of Sugar Crops held during 23-25 June 2014 in Coimbatore, pp. 192-194.
Ramasubramanian T, Ramaraju K, Pandey SK (2015a) DNA barcode of Melanaphis sacchari (Zehntner). Research paper presented in the International Symposium on New Perspectives in Modern Biotechnology held during 23-25 March 2015 in Puducherry, Abstract book p. 26.
Ramasubramanian T, Ramaraju K, Pandey SK (2015b) DNA barcodes of sugarcane Aleyrodids. International Sugar Journal 117(1395): 206-211.
Ramasubramanian T, Ramaraju K, Nirmala R (2016). COI-gene based species diagnostic kit for sugarcane scale insect, Melanaspis glomerata Green (Homoptera: Diaspididae). Sugar Tech 18(4): 441-446.
Rebijith KB, Asokan R, Krishna Kumar NK, Srikumar KK, Ramamurthy VV, Shivarama Bhat P (2012) DNA barcoding and development of species-specific markers for the identification of tea mosquito bugs (Miridae: Heteroptera) in India Environmental Entomology 41(5): 1239-1245.
Rebijith KB, Asokan R, Krishna Kumar NK, Krishna V, Chaitanya BN, Ramamurthy VV (2013) DNA barcoding and elucidation of cryptic aphid species (Hemiptera: Aphididae) in India. Bulletin of Entomological Research 103(5): 601-610.
Sohn JC, Park KT, Cho S (2015) A taxonomic review of Schoenobiinae (Lepidoptera: Crambidae) from Korea. Journal of Asia-Pacific Entomology 18: 131-137.
Tembe S, Shouche Y, Ghate, HV (2014) DNA barcoding of Pentatomomorpha bugs (Hemiptera: Heteroptera) from Western Ghats of India. Meta Gene 2:737-745.
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