SSR genotyping of germplasm collections of Saccharum edule Hassk.
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Authors
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K Chandran
ICAR - Sugarcane Breeding Institute
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A.K Shibina
ICAR - Sugarcane Breeding Institute
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M Nisha
ICAR - Sugarcane Breeding Institute
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Maya Lekshmi
ICAR - Sugarcane Breeding Institute
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R ArunKumar
Abstract
Saccharum edule Hassk.is cultivated for its aborted edible inflorescence in pacific Islands. The most accepted classification of Saccharum comprises of six species including S.edule, but the status and origin of S.edule was in debate due to its similarities with S.robustum.Though molecular studies have been widely utilized in sugarcane systematic; S.edule was not included in any of these studies. In this study, 17 accessions of S.edule along with five other species of Saccharum were characterized using eight SSR markers. Multiple bands were observed for all the primers used and the highest PIC value highest number of bands were observed for the primer SMC334BS and NKS5 was revealing the least polymorphism. S.spontaneum and S.robustum showed lesser number of bands across the primers. In cluster analysis three main clusters were formed and the other species clones except S.robustum found to cluster in one group along with six S.edule accessions and the S.robustum with another clone of S.edule. Probably the less number of accession used in other species might have resulted in such grouping. However, the grouping of accession in three distinct clusters indicates the wide genetic variability within the collection
References
Brandes EV, Sartoris GB, Grassl CO (1939) Assembly and evaluation of wild forms of sugarcane and closely related plants. Proceedings of International Society of Sugar Cane Technologists 6: 128-153.
Brown S J, Schnell RJ, Power E J, Stephanie L. Douglas, David N. Kuhn (2007) Analysis of clonal germplasm from five Saccharum species: S. barberi, S. robustum, S. officinarum, S. sinense and S. spontaneum. A study of inter- and intra species relationships using microsatellite markers. Genetic Resources and Crop Evolution54:627–648.
Chandran K (2011) In Vitro Regeneration of Saccharum edule from Immature Inflorescence. Sugar Tech 13(2):170–173.
Chandran K, Nisha M, Mahesh P (2014) Saccharum eduleHassk.an under- exploited genetic resource of sugarcane. In proceedings of International Seminar on Biodiversity, Bioresources and Biotechnology 30-31 Jan, 2014, Mysore Association for the Advancement of Biodiversity Science, UAS, Karnataka. p 27.
Chen PH, Pan YB, Chen RK., Xu LP, Chen YQ (2009) SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum spp. hybrids) from breeding programs in China and other countries. Sugar Tech 11(4): 347-354.
Chowing A (1963) Proto-Melanesian plant names. In Plants and the Migrants of Pacific Poples, A symposium. (ed. Jacques Barrau ), Bishop Museum Press, Honolulu:39-44.
Cordeiro GM, Taylor GO, Henry RJ (2000) Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploidy species.Plant Science 155: 161–168.
Daniel J, Roach BT (1987) Taxonomy and evolution. In: Sugarcane improvement through breeding, (ed. DJ Heinz). Elsevier, Amsterdam: 7–84.
Grassl CO (1976) Introgression between Sccharum and Miscanthus in New Guinea and Pacific. Proceedings of International Society of Sugar Cane Technologist 12: 995-1003.
JanakiAmmal EK (1941) Intergeneric hybrids of Saccharum. Journal of Genetics 41:217-253
Jannoo N, Forget L, Dookun K (2001) Contribution of microsatellites to sugarcane breeding programme in Mauritius.Proceedings of International Society of Sugar Cane Technologist 24: 22-27.
Lennoux CG (1939) Sugarcane collection in New Guinea during 1937.Proceedings of International Society of Sugar Cane Technologist 6:171–182.
Linda Mondini, ArshiyaNoorani and Mario A. Pagnotta (2009) Assessing Plant Genetic Diversity by Molecular Tools.Diversity 1: 19-35.
Mudaliar T (2007) Duruka (Saccharum edule L) Growing in Fiji; Technical Bulletin Issue No: 4. Ministry of Primary industries Information and communication division.
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321-4325.
Neil C Glynn, Kay McCorkle, Jack C Comstock (2009) Diversity among mainland USA sugarcane cultivars examined by SSR genotyping. Journal of the American Society of Sugar Cane Technologists 29: 36-52
Olufowote JO, Xu YB, Chen XL, Park WD, Beachell HM., Dilday, RH., Goto M, McCouch, SR (1997) Comparative evaluation of within-cultivar variation of rice (Oryza sativa L.) using microsatellite and RFLP markers. Genome40: 370–378.
Pan YB, Cordeiro GM, Richard EP Jr, Henry RJ (2003) Molecular genotyping of sugarcane clones with microsatellite DNA markers. Maydica 48: 319-329.
Parthiban S, Govindaraj P, Senthilkumar S. (2018)Comparison of relative efficiency of genomic SSR and EST-SSR markers in estimating genetic diversity in sugarcane. Biotech 8 (3):144. doi: 10.1007/s13205-018-1172-8.
Premachandran MN (2006) Cauliflower gene in sugarcane?. Current Science 91(6): 750-751
Saraswati P, Mawikere NL, Djuuna IAF, Pakiding F (2013) The diversity and cultivation system of Saccharum edule and its role as an edible plant source in Papua, Indonesia. ActaHorticulturae 979; 373-380. DOI: 10.17660/ActaHortic.2013.979.40
Selvi A, Nair NV, Noyer JL, Singh NK, Balasundaram N, Bansal KC, Koundal KR, Mohapatra T (2006) AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum andErianthus. Genetic Resources and Crop Evolution 53: 831–842.
Ved Kumar Mishra, Vineeta Singh, Prashant Kumar Mishra , Ram Kushal Singh, Naveen Dwivedi, ShubhaDwivedi, Ashwani Kumar Singh and PrashantAnkur Jain (2015) Screening of SSR marker for sugar and sugar related traits. Advances in Applied Science Research 6(4):202-209.
Zhi-Nian Deng, Yuan-Wen Wei, Zhong-Liang Chen, Cheng-Mei Huang, You-Qiang Pan, Wei-Li Lü, Cui-Xian Qin, Hai-Bo He, Yuan-Yuan Guo, Hai-Bin Luo, Li-Tao Yang and Yang-Rui Li (2015) SSR marker based genetic diversity in sugarcane varieties (lines) developed in Guangxi, China .International Journal of Development Research 5 : (1) 3078-3084.
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