PHYLOGENETIC ANALYSIS OF COLLETOTRICHUM FALCATUM ISOLATES CAUSING RED ROT IN SUGARCANE
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Authors
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P. Malathi
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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R. Viswanathan
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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A. Ramesh Sundar,
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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P. Padmanaban
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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N. Prakasam
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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D. Mohanraj
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
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R. Jothi
Sugarcane Breeding Institute
Coimbatore – 641007, Tamil Nadu, India
Abstract
The fungal pathogen Colletotrichum falcatum, causing red rot, exhibits enormous variation. Representative isolates of C. falcatum collected from major red rot endemic regions in the country were characterized by cultural, pathological and molecular methods using the 5.8s-ITS region. Phylogenetic analysis of ITS sequences confirmed the divergent and unique nature of C. falcatum from other Colletotrichum spp. and separated the Indian isolates under three distinct molecular groups as Group I, II, and III. Although the dark coloured, non-sporulating least virulent types similar to isolates of other countries were distinguished in Group III, diversity among Group I and II was not much supported by cultural or pathogenicity patterns. However, overall correlation could be made with respect to phenotypic and genotypic characters based on their predominance in each group. This is the first attempt to group a large collection of C. falcatum isolates using molecular approach and correlate with other traits.References
Abang MM, Winter S, Green KR, Hoffmann P, Mignouna HD, Woil GA (2002) Molecular identification of Colletotrichum causing yam anthracnose in Nigeria. Plant Pathology 51: 63-71.
Abbot t EV (1935) Physiologic special izat ion in Colletotrichum falcatum. Proc. Intern. Soc. Sugar Cane Technologists, 5: 730-736.
Edgerton CW, Moreland CC (1920) Effect of fungi on the germination of sugarcane. Louisiana Agri. Expt. Sta. Bull., 169.
Freeman S, Minz D, Jurkevitch E, Maymon M, Shabi E (2000) Molecular analyses of Colletotrichum species from almond and other fruits. Phytopathology 90: 608-614.
Freeman S, Minz D, Maymon M, Zveibil A (2001) Genetic diversity within Colletotrichum acutatum sensu Simmonds. Phytopathology 91: 586-592.
Guerber JC, Liu B, Correll C, Johnston PR (2003) Characterization of diversity in Colletotrichum acuttum sensu lato by sequence analysis of two introns, mtDNA and intron RFLPs and mating compatibility. Mycologia 3: 872-895.
Gupta SC, Singh MP, Upadhyay UC (1980) A new biotype of Colletotrichum falcatum Went. Current Science 49: 600-601.
Hsiang T, Goodwin PH (2001) Ribosomal DNA sequence comparisons of Colletotrichum graminicola from turfgrasses and other hosts. European J. Pl. Pathol. 107: 593-599.
Johnston RP, Jones D (1997) Relat ionships among Colletotrichum isolates from fruit rots assessed using rDNA sequences. Mycologia 89: 420-430.
Malathi P, Viswanathan R, Jothi R (2006) Specific adaptation of Colletotrichum falcatum pathotypes to sugarcane cultivars. Sugar Tech. 8(1): 52-56.
Malathi P, Viswanathan R, Ramesh Sundar A, Prakasam N, Padmanaban P, Jothi R, Renuka Devi SR, Poongothai M (2010) Variability among Colletotrichum falcatum pathotypes used for screening red rot resistance in sugarcane. Sugar Cane International 28(2): 47-52.
Martinz-Culebras PV, Querol A, Surez-Fernandez MB, Garcia-Lopez MD, Barrio E (2003) Phylogenetic relationships among Colletotrichum pathogens of strawberry and design of PCR primers for their identification. Journal of Phytopathology 151: 135-143.
Mohanraj, D., Kumaresan S., and Sreenivasan, T. V. (2002). Molecular characterizat ion of isolates of the sugarcane red rot pathogen Colletotrichum falcatum Went. Indian Phytopathol. 55(2): 147-151.
Padmanaban P, Mohanraj D, Viswanathan R, Rao MM, Prakasam N, Jothi R, Alexander KC (1996) Differential interaction of sugarcane clones to pathotypes of Colletotrichum falcatum Went. Sugar
Cane 4: 16-20.
Pers N, Kuramae AR, Dias EE, De Souza LD (2002) Identification and characterization of Colletotrichum spp. affecting fruit after harvest in Brazil. J. Phytopathology 150: 128-134.
Rafay SA, Singh VB (1957) A new strain of Glomerella tucumanensis. Curr. Sci. 26: 19-20.
Raid RN, Lentinil RS (2002). Sugarcane Red-rot Disease, Sugarcane Handbook, Florida: UF/IFAS Publication SS-AGR-206.
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. National Acad. Sci. USA 81: 8014-8019.
Sreenivasaprasad S, Mills PR, Brown AE (1994) Nucleotide sequence of the rDNA spacer enables identification of isolates of Colletotrichum as C. acutatum. Mycol. Res. 98: 186-188.
Sreenivasaprasad S, Mills PR, Meehan BM, Brown AE (1996a) Phylogeny and systemat ics of 18 Colletotrichum species based on ribosomal DNA spacer sequences. Genome 39:499-512.
Srinivasan KV, Bhat NR (1961) Red rot of sugarcane: Criteria Red rot disease for grading resistance. J Indian Bot Soc. 40:566-577.
Suman A, Sunita Lal, Shasany A. K, Asha Gaur and Pushpa Singh. (2005). Molecular assessment of diversity among pathotypes of Colletotrichum falcatum prevalent in sub-tropical Indian sugarcane. World J. Microbiol. Biotechnol. 21: 1135-1140.
Talhinhas P, Sreenivasaprasad S, Neves-Martins, J, Olivera H (2002). Genetic and morphological charcterization of Colletotrichum acuttum causing anthracnose in lipins. Phytopathology 92: 986-996.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic
Acids Res. 25: 4876-4882.
Valerio HM, Resende MA, Weikert-Oliveira RCB, Casela CR (2005) Virulence and molecular diversity in Col letotrichum graminicola from Brazi l . Mycopathologia 159: 449-459.
Viswanathan R (2010). Plant Disease: Red Rot of Sugarcane, Anmol Publishers, New Delhi, India, p 306.
Viswanathan R, Padmanaban P, Mohanraj D, Jothi R (2000). Indirect-ELISA technique for the detection of the red rot pathogen in sugarcane (Saccharum spp hybrid) and resistance screening. Indian J. Agric. Sci.
: 308-11.
Viswanathan R, Malathi P, Padmanaban P (2003) Variation in sugarcane red rot pathogen Colletotrichum falcatum Went. In: Frontiers of Fungal Diversity in India. (Eds G. P. Rao, C. Manoharachari, D. J. Bhat, R. C. Rajak and T. N. Lakhanpal), International Book Distributing Co, Lucknow, 639-667.
Whitelaw-Weckert MA, Curtin SJ, Haung R, Steel CC, Blanchard CL, Roffey PE (2007). Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia.
Plant Pathology 56: 448-563.
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