RHIZOSPHERE COMPETENCE OF THREE ENTOMOPATHOGENIC FUNGI IN RELATION TO HOST PLANT AND INTER-SPECIFIC INTERACTION
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Authors
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N. Geetha
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
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D. Nithya
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
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K. Hari
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
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M. Preseetha
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
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K. Subadra Bai
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
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G. Santhalakshmi
Sugarcane Breeding Institute
Coimbatore 641007, Tamil Nadu, India
Abstract
Three commonly used entomopathogenic fungi (EPF) in sugarcane ecosystem, namely Beauveria bassiana (Balsamo) Vuillemin, Beauveria brongniartii (Saccardo) Petch and Metarhizium anisopliae (Metchnikoff) Sorokin were assessed for their ability to sustain themselves in the rhizosphere in the presence or absence of competition with other fungi, either native or inoculated. In the first of two studies, the inoculated EPFs applied singly were recovered from rhizosphere of five dicots and five monocot crops from sterilized soil as well as unsterilized soil. In the second study, the efficacy of the EPF to survive in the rhizosphere of sugarcane with or without competition from either of the other two EPF or two nematophilic fungi or two antagonistic fungi or two soil saprophytes or sugarcane wilt pathogen Fusarium sp. in sterilized or unsterilized soil was estimated. Results of the first study indicated that in sterilized soil medium, the rhizosphere of okra (82.2%) was the most favourable for survival of EPF which was comparable with most monocots tested. Beauveria bassiana (75.0%) and M. anisopliae (79.0%) were more efficient than B. brongniartii (66.3%) regardless of plant species. The lowest spore harvest was from larvae rapped in rhizosphere of brinjal (3.88 x 107/larva) followed by that from cotton rhizosphere (5.55x107/larva). In the second study, in sugarcane rhizosphere in sterilized medium, the mortality of the larvae caused by B. bassiana was synergized by M. anisopliae (96.7%) and significantly higher than all other treatments. In case of B. brongniartii, mortality rates were most affected by fungal antagonists and Fusarium sp. (50.0-53.3%) while M. anisopliae was affected by Trichoderma harzianum (53.3%). Sporulation of Beauveria spp. was consistently affected by Penicillium sp./Aspergillus sp. while most species were competitive with M. anisopliae. In both the studies, only M. anisopliae spores could be recovered from unsterilized soil.
References
Acevedo JPM, Samuels RI, Machado IR, Dolinski C (2007) Interactions between isolates of the entomopathogenic fungus Metarhizium anisopliae and the entomopathogenic nematode Heterorhabditis bacteriophora JPM4 during infection of the sugar cane borer Diatraea
saccharalis (Lepidoptera: Pyralidae) Journal of Invertebrate Pathology 96:187-192.
Barua L, Bora BC (2009) Compatibility of Trichoderma harzianum and Pseudomonas fluorescens against Meloidogyne incognita and Ralstonia
solanacearum complex on brinjal. Indian Journal of Nematology 39(1): 29-34.
Bidochka MJ (2001) Monitoring of the fate of biocontrol fungi. In: Fungi as Biocontrol Agents: Progress, Problems and Potential (ed. T.M. Butt, C.W. Jackson, and N. Magan) Oxon: CABI Publishing: 193–218.
Bruck DJ (2010) Fungal entomopathogens in the rhizosphere. BioControl 55:103–112.
Dhoj YGC, Keller S, Nagel P (2008). Occurrence of insect pathogenic fungi in Nepal with especial reference to Beauveria bassiana and
Metarhizium anisopliae. Journal of Plant Protection Society 1: 106-114.
Dixon GR, Tilston EL (2010) Soil microbiology and sustainable crop production, (Ed) Springer, Newyork: 340.
Easwaramoorthy S, Srikanth J, Santhalakshmi G, Geetha N (2004) Laboratory and field studies on Beauveria brongniartii (Sacc.) Petch. against Holotrichia serrata F. (Coleoptera: Scarabaeidae) in sugarcane. Proc A Conv Sug Technol Ass India 66:A3-A19
Goble T, Dames J, Hill PM, Moore S (2010) The effects of farming system, habitat type and bait type on the isolation of entomopathogenic fungi from citrus soils in the Eastern Cape Province,
South Africa. BioControl 55:399-412.
Hintz WE, Becker EM, Shamoun SF (2001) Development of genetic markers for risk assessment of biological control agents. Canadian Journal of Plant Pathology 23:13-18.
Inglis GD, Goettel MS, Butt TM, Strasser H (2001) Use of Hyphomycetous fungi for managing insect pests. In: Fungi as Biocontrol Agents (ed. T.M Butt, C. Jackson, and N. Magan) Wallingford: CAB International 23–69.
Jash S, Pan S (2007) Variability in antagonistic activity and root colonizing behaviour of Trichoderma isolates Journal of Tropical Agriculture 45: 29–35.
Meyling, NV (2007) Methods for isolation of entomopathogenic fungi from the soil environment. Laboratory manual, Department of Ecology, Faculty of Life Sciences, University of Copenhagen,Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark: http://orgprints.org/
/1/11200.pdf
Ripoll MP, Fang CA, Wang S, Posada FJ, St Leger R (2011) The rhizosphere-competent entomopathogen Metarhizium anisopliae
expresses a specific subset of genes in plant root exudate Microbiology 157:47-55.
Sookar P, Bhagwant S, Ouna EA (2008) Isolation of entomopathogenic fungi from the soil and their pathogenicity to two fruit fly species
(Diptera:Tephiritidae). Journal of Applied Entomology 132:778-788.
Schmidt EL (1979) Initiation of plant root-microbe interactions. Annual Review of Microbiology 33: 355–376.
Vega FE, Goettel MS, Blackwell M, Chandler D, Jackson MA, Keller S, Koike M, Maniania NK, Monzo´n A, Ownley BH, Pell JK, Rangel DEN, Roy HE (2009) Fungal entomopathogens: new insights
on their ecology. Fungal Ecology 2:149–159.
Zimmermann G (1986) The Galleria bait method for detection of entomopathogenic fungi in soil.Journal of Applied Entomology 102: 213-215.
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