AMENABILITY OF THE SUGARCANE VARIETY 2005 T 16 TO SHOOT TIP CULTURE


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Authors

  • B. Jagadeesh Agricultural Research Station, Perumallapalle, Tirupati 517505, Andhra Pradesh, India
  • M. Hemanth Kumar Agricultural Research Station, Perumallapalle, Tirupati 517505, Andhra Pradesh, India
  • M. Reddi Shekhar Agricultural Research Station, Perumallapalle, Tirupati 517505, Andhra Pradesh, India
  • P. Sudhakar Agricultural Research Station, Perumallapalle, Tirupati 517505, Andhra Pradesh, India

Abstract

The protocol for regeneration of plantlets through in vitro culture using shoot tip was established for a pre-released early maturing sugarcane variety, 2005T16. Inoculation of shoot tip explants, surface sterilized with 10% sodium hypochlorite for 20 min on MS medium containing 3.0 mg l-1 BAP, 2.0 mg l-1 IAA and 2.0 mg l-1 kinetin led to axillary bud initiation and establishment. The highest multiple-shoot induction of 88.89% frequency within 12.3 days was also observed on the same medium with production of 12.3 multiple-shoots per axillary shoot. The best rooting of 77.78% and 6.8 roots per shoot having a mean length of 3.76 cm were recorded for the shootlets on half strength MS medium supplemented with 6 mg l-1 NAA. During acclimatization the highest survival percentage was observed in vermicompost: soil: sand mixture in 1:1:1 ratio.

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Submitted

20-03-2020

Published

21-03-2020

Issue

Vol. 1 No. 2 (2011)

Section

Short Communication

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How to Cite

Jagadeesh, B., Hemanth Kumar, M., Reddi Shekhar, M., & Sudhakar, P. (2020). AMENABILITY OF THE SUGARCANE VARIETY 2005 T 16 TO SHOOT TIP CULTURE. Journal of Sugarcane Research, 1(2). https://epubs.icar.org.in/index.php/JSR/article/view/99278