Effect of medicinal plant extracts on mycelial growth of Pleurotus florida
A study was conducted at Department of Plant Pathology to evaluate the effect of medicinal amendments in edible species of oyster mushroom, Pleurotus florida. The medicinal plants viz., Adathoda (Justicia vasica), neem (Azadirachta indica) and ocimum Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â (Ocimum sanctum) were used as amendments. The dried and powdered leaves of the medicinal plants were used for assessing their efficacy. In vitro evaluation was carried out with the medicinal amendments using poisoned food technique. Adathoda (Justicia vasica), Neem (Azadirachta indica) and Ocimum (Ocimum sanctum) was amended in potato dextrose agar medium (PDA) at 250, 500 and 1000 ppm concentrations along with control. The experiment was laid out in Completely Randomized Design with ten treatments and four replication. Nature of mycelial growth, rate of mycelial growth (cm day-1) and days taken for completion of growth in petri dish were observed. The mycelium of P. florida in amended medium produced white sparse growth with fluffy centre later turning to whitish fluffy growth with regular margin whereas in control plate the mycelium was with white sparse growth later turning to white cottony with regular margin on full growth in petri dish. The rate of mycelial growth was highest in control (1.6 cm day-1). The days taken for complete mycelial growth in petri dish ranged from 7 to 8 among the various amendments used. The addition of medicinal amendments was not inhibitory to the mycelial growth of P.florida indicating its use in biomedicinal enrichment with pharmaceutical properties.Â
Alam, N., R. Amin, A. Khan, I. Ara, S. Mi Ja and W. L. Min. 2009. Comparativeeffects of oyster mushroom on lipid profile, liver and kidney function in hypercholesterolemic rats. Mycobiology 37: 37-42.
Carrasco-Gonzalez, J. A., S. O. Serna-Saldivar and J. A. Gutierrez-Uribe. 2017. Nutritional composition and nutraceutical properties of the Pleurotus fruiting bodies: potential use as food ingredient. Journal of Food Composition and Analysis 58: 69-81.
Chang, S.T. and J.A. Buswell. 1996. Mushroom nutraceuticals. World Journal of Microbiology and Biotechnology 12(5): 473-476.
Chang, S. T. and P. G. Miles. 1992. Mushroom biologyâ€”a new discipline. Mycologist6(2): 64â€“65.
Eswaran, A. and R. Ramabadran. 2000. Studies on some physiological, cultural and postharvest aspects of oyster mushroom, Pleurotus eous (Berk.) Sacc. Tropical Agriculture and Research 12: 360-374.
Giavasis, I. 2014. Bioactive fungal polysaccharides as potential functional ingredients in food and nutraceuticals. Current Opinion in Biotechnology 26:162â€“173
Inam-ul-haq, M., N. A. Khan, M. A. Khan, N. Javed, R. Binyamin and G. Irshad. 2010. Use of medicinal plants in different composts for yield improvement of various strains of oyster mushroom. Pakistan Journal of Botany 42(5): 3275-3283.
Kumar, S., G. Singh, Mohit and J. P. Kannaujia. 2019. In-vitro evaluation of mycelial growth of Pleurotus sapidus against different botanicals. Journal of Pharmacognosy and Phytochemistry 8(1): 655-657.
Moon, B. and Y. M. Lo. 2014. Conventional and novel applications of edible mushrooms in todayâ€™s food industry. Journal of Food Processing Preservation 38(5): 2146â€“2153.
Nene, Y. L. and P. N. Thapliyal. 1993. Fungicides in Plant Disease Control (3rd Ed.). Oxford and IBH publishing Co. Pvt. Ltd. 691p.
Owaid, M. N., S. Jaloot and D. M. Ahmed. 2019. Influence of Ficus carca and Olea europaea leaves extracts on the mycelial growth of mushrooms in vitro. Acta Ecologica Sinica 39(1): 36-41.
Pandey, S., R. Prasad, S. Kumar and M. Singh. 2021. Effect of different botanical extract on mycelial growth of Pleurotus florida (Mont.). Journal of Medicinal Plants Studies 9(4): 42-43.
Shah, S., S. Nasreen and N. A. Munshi. 2011. Evaluation of some botanicals in controlling green mold (Trichoderma harzianum) disease in oyster mushroom cultivation. International Journal of Botany 7(3): 209-215.
Wink, M. 2015. Modes of action of herbal medicines and plant secondary metabolites. Medicines 2 (3): 251-286
How to Cite
I/We have taken permission from the copyright holder to reproduce the matter not owned by me and acknowledged the source.
I/We permit editors to publish the said paper in the journal or in any other means with editorial modification, if any.
In case of publication of the article in the journal,
I/We hereby assign copyright to the Mushroom Research Journal for its publication in any form/language including all media (print and electronic, or presently unknown), and exclusive right to use the matter for the life of the work (no time restriction on re-use of matter).Â