Single spore isolation and hybridization of Calocybe spp.

Abstract views: 114 / PDF downloads: 157


  • VIJETH S Department of Plant Pathology, College of Agriculture, Vellayani (Kerala)


A study was conducted at Department of Plant Pathology for the development of improved strain(s) of Calocybe spp. with desirable traits by interspecific hybridization. Milky white mushroom (C. indica) and St. George’s mushroom (C. gambosa) were the species used for hybridization. Pure culturing and morphological characterization of sporocarp of Calocybe spp. was done. In vitro evaluation of both the species for mycelial characteristics, growth rate, colony colour, days taken for completion of full growth were recorded. Single spore isolation was done through serial dilution technique. Hybridisation of single spores was done by dual culture technique using hyphal anastomosis for the development of hybrids. Of the 121 crosses done 22 were compatible and 79 crosses incompatible.

Keywords: Calocybe indica, C. gambosa, Interspecific hybridization, mycelial growth rate.

Author Biography


    Assistant Professor and PI (AICRP on Mushrooms)

    Dept of Plant Pathology, College of Agriculture , Vellayani



Anitha, R.1998. Strain improvement in Oyster mushroom (Pleurotus spp.). M.Sc.(Ag) thesis, Kerala Agricultural University, Thrissur, 96p.

Bahukhandi, D. and R. K. Sharma. 2012. Interspecific hybridization between Pleurotus species. Indian Phytopathology 55(1): 61- 66.

Chakraborty, U. and S. R. Sikdar. 2010. Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom. World Journal of Microbiology and Biotechnology 26(2): 213-225.

Gogoi, R., Y. Rathaiah and T. R. Borah. 2019. Mushroom Cultivation Technology. Scientific Publishers, Jodhpur, 124p.

Gupta, B., B. P. N. Reddy and A. S. Kotasthane. 2011. Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement. World Journal of Microbiology and Biotechnology. 27(1): 1-9.

Heera, G. 2006. Strain improvement and production technology of milky mushroom (Calocybe indica P&C.). Ph.D(Ag) thesis, Kerala Agricultural University, Thrissur,181p.

Jandaik, C. L. 1997. History and Development of Pleurotus cultivation in the world and future prospects. Advances in Mushroom Biology and Production (eds. Rai, R. D., Dhar, B. L., Verma, R. N), Mushroom Research Station, Solan, pp.181-192.

Kaur, J., H. S. Sodhi, S. Kapoor, P. K. Khanna and R. K. Jaswal. 2011. Strain improvement of specialty mushroom, Calocybe indica, through mutagenesis. Applied Biological Research 13(2): 62-69.

Krishnamoorthy, A. S. and V. Balan. 2015. A comprehensive review of tropical milky white mushroom (Calocybe indica P&C). Mycobiology 43(3): 184- 194.

Lakshmipathy, G., A. Jayakumar and S. P. Raj. 2012. Optimization of growth parameters for increased yield of the edible mushroom Calocybe indica. African Journal of Biotechnology, 11(30): 7701-7710.

Petersen, R. H. and G. S. Ridley. 1996. A New Zealand Pleurotus with Multiple-Species Sexual Compatibility. Mycologia 88(2): 198-207.

Phutela, U.G. and R. P. Phutela. 2012. Effect of physical and chemical factors on growth of Calocybe indica (P & C). International Journal of Advanced Life Science 2(1): 8-16.

Purkayastha, R. P. and A. Chandra. 1974. New species of edible mushroom from India. Transactions of British Mycological Society 62(2): 415-418.

Subbiah, K. A. 2014. Biodiversity exploration of milky mushroom (Calocybe Indica P&C)- Concept to Commercialization. In: Proceedings of 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8), 19-22 November, 2014, New Delhi. ICAR-Directorate of Mushroom Research, Solan, pp. 490-495.

Zied, D. C. and A. Pardo-Gimï. 2017. Edible and medicinal mushrooms: technology and applications. John Wiley & Sons, United Kingdom, 541p.









How to Cite

Single spore isolation and hybridization of Calocybe spp. (2022). Mushroom Research, 31(1), 49-60.