Growth of oyster mushroom using sawdust and agriculture waste as substrates

Abstract views: 599 / PDF downloads: 380


  • ALYSSA ADAMS University of Guyana
  • DIANA SEECHARRAN University of Guyana
  • ABDULLAH ANSARI University of Guyana


Mushroom culture is an important micro-industrial source of protein in developing countries such as Guyana.  This study investigated the growth and mineral composition of Pleurotus ostreatus (oyster mushroom) using rice straw, grass clippings [Bermuda grass (Cynodon dactylon)] and sawdust as substrate materials. Growth was only successful in the treatment that contained rice straw as substrate, whereas no growth was obtained from substrates that contain grass clippings and sawdust. N, P, K, Ca, Fe, Cu, Mn and Mg were present in the oyster mushroom samples and the spent mushroom substrates.

Author Biographies

  • ALYSSA ADAMS, University of Guyana
  • DIANA SEECHARRAN, University of Guyana


    Senior Lecturer 

  • ABDULLAH ANSARI, University of Guyana




Abdel-Azeem AM. 2010. The history, fungal biodiversity, conservation, and future perspectives for mycology in Egypt. IMA fungus 1(2): 123-42.

Adebayo EA, Martinez-Carrera D. 2015. Oyster mushrooms (Pleurotus) are useful for utilizing lignocellulosic biomass. Afr J Biotechnol 14(1): 52-67.

Ahlawat OP, Sagar MP. 2007. Management of spent mushroom substrate. National Research Centre for Mushroom, Indian Council of Agricultural Research.

Ahmed M, Abdullah N, Ahmed KU, Bhuyan MH. 2013. Yield and nutritional composition of oyster mushroom strains newly introduced in Bangladesh. Pesqui Agropecu Bras 48(2): 197-202.

Ahmed MO, Abdullah N, Nuruddin MM. 2016. Yield and nutritional composition of oyster mushrooms: An alternative nutritional source for rural people. Sains Malaysiana 45(11): 1609-15.

Alananbeh KM, Bouqellah NA, Al Kaff NS. 2014. Cultivation of oyster mushroom Pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in Saudi Arabia. Saudi J Biol Sci 21(6): 616-25.

Ansari AA. 2010. Vermitech: an innovation in organic solid waste management. Environmental Research and Technology 737.

Ashraf J, Ali MA, Ahmad W, Ayyub CM, Shafi J. 2013. Effect of different substrate supplements on oyster mushroom (Pleurotus spp.) production. Food Sci Technol 1(3): 44-51.

Bhattacharjya DK, Paul RK, Miah MN, Ahmed KU. 2015. Comparative study on nutritional composition of oyster mushroom (Pleurotus ostreatus Fr.) cultivated on different sawdust substrates. Bioresearch Communications-(BRC) 1(2): 93-8.

Brenneman JA, Guttman MC. 1994. The Edibility & Cultivation of the Oyster Mushroom. The American Biology Teacher 1: 291-3.

Campos JA, Tejera NA, Sánchez CJ. 2009. Substrate role in the accumulation of heavy metals in sporocarps of wild fungi. Biometals 22(5): 835-41.

Daba AS, Kabeil SS, Botros WA, El-Saadani MA. 2008. Production of mushroom (Pleurotus ostreatus) in Egypt as a source of nutritional and medicinal food. World J Agric Sci 4(5): 630-4.

Deepalakshmi K, Sankaran M. 2014. Pleurotus ostreatus: an oyster mushroom with nutritional and medicinal properties. J Biochem Technol 5(2): 718-26.

Dima GA, Popescu IV, Stihi CL, Oros CA, Dinu SE, Manea L, Vlaicu GH. 2006. Fe, Mn and Zn concentrations determination from Ialomita River by atomic absorption spectroscopy. Rom J Phys 51(5/6): 667.

Hoa HT, Wang CL, Wang CH. 2015. The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology 43(4): 423-34.

Ibekwe VI, Azubuike PI, Ezeji EU, Chinakwe EC. 2008. Effects of nutrient sources and environmental factors on the cultivation and yield of oyster mushroom (Pleurotus ostreatus). Pak J Nutr 7(2): 349-51.

Isaac RA, Kerber JD. 1971. Atomic absorption and flame photometry: Techniques and uses in soil, plant, and water analysis. Instrumental methods for analysis of soils and plant tissue 1: 17-37.

Josephine RM. 2014. A review on oyster mushroom (Pleurotus spp). Int J Adv Biotechnol Res 1: 7-9.

KalaÄ P. 2010. Trace element contents in European species of wild growing edible mushrooms: a review for the period 2000–2009. Food Chem 122(1): 2-15.

Kurtzman RH, Zadrazil F. 1982. Physiological and taxonomic considerations for cultivation of Pleurotus mushrooms. In Chang ST, Quimio TH (Eds.) Tropical Mushrooms: Bio- logical Nature and Cultivation Methods. Chinese University Press. Hong Kong 299-348.

Mandeel QA, Al-Laith AA, Mohamed SA. 2005. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World J Microbiol Biotechnol 21(4): 601-7.

Mane VP, Patil SS, Syed AA, Baig MM. 2007. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer. J Zhejiang Univ Sci B 8(10): 745-51.

Nunes MD, da Luz JM, Paes SA, Ribeiro JJ, da Silva MD, Kasuya MC. 2012. Nitrogen supplementation on the productivity and the chemical composition of oyster mushroom. J Food Res 1(2): 113.

Ouzouni PK, Petridis D, Koller WD, Riganakos KA. 2009. Nutritional value and metal content of wild edible mushrooms collected from West Macedonia and Epirus, Greece. Food Chem 115(4): 1575-80.

Pathmashini L, Arulnandhy V, Wijeratnam RS. 2010. Efficacy of different spawn types on sawdust media. Trop Agr Res Ext 11.

Petisleam T, Popescu IV, Ciupina V, Belc M. 2007. Considerations regarding Cu and Ni determination from marine environmental samples of black-sea using FAAS. Rom J Phys 52(3/4): 441.

Royse DJ, Baars J, Tan Q. 2017. Current overview of mushroom production in the world. Edible and medicinal mushrooms: technology and applications In: Zied, D. C., and Pardo-Giménez, A. (Eds.). Edible and Medicinal Mushrooms: Technology and Applications, John Wiley and Sons Ltd 5: 5-13.

Seecharran D, Ansari AA, Subramanian G, Nankishore A, Sambhu H, Punu B. 2018. An investigation into culture and propagation of edible mushrooms on different organic substrates. Mushroom Res 27(1): 45-53.

Tisdale TE. 2014. Cultivation of the oyster mushroom (Pleurotus sp.) on wood substrates in Hawaii [Dissertation].

Viriato V, Mäkelä MR, Kowalczyk JE, Ballarin CS, Loiola PP, Andrade MC. 2021. Organic residues from agricultural and forest companies in Brazil as useful substrates for the cultivation of the edible mushroom Pleurotus ostreatus. Lett Appl Microbiol 74(1): 44-52.

Wagner RE, Kotas W, Yogis GA. 1994. Guide to Environmental Analytical Methods, Genium. Schenectady, NY.









How to Cite

Growth of oyster mushroom using sawdust and agriculture waste as substrates. (2022). Mushroom Research, 31(1), 73-80.