Cellulolytic and lignolytic enzyme activity by different strains of Humicola insolens on six different compost formulation at different pH
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Authors
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ASHUTOSH PATHAK
ASSISTANT PROFESSOR
DEPARTMENT OF LIFE SCIENCES
ITM UNIVERSITY
GWALIOR(MP)
Abstract
In present investigation extracellular secretion of cellulolytic and lignolytic enzymes of four different strains of H. insolens were assayed on six different compost formulations at different pH and highest cellulolytic and lignolytic activity was exhibited in formulation-6 (F-6) in terms of xylanase and CMCase having wheat bran in medium followed by formulation-5 and 2 (F-5 and F-2) having cotton seed meal and cotton seed cake. Highest laccase activity was observed in medium supplemented with nitrogen compounds viz Cotton seed meal and cotton seed cake (F-5 and F-2) from strain-1 followed by strain-2 and 3. Least activity was observed in strain-4. Thus, from study it was concluded that formulation (F-6) containing wheat bran supported maximum liberation of these enzymes of strain-1. Therefore strain-1 of the test fungus along with formulation F-6 will be chosen best for composting. Â
Keywords: Humicola insolens, compostimg, cellulase, laccaseReferences
. Bilay, V. (1995). Interaction of thermophilic fungi from mushroom compost on agar medium. In: Sci. Cultiv. Edible Fungi. Elliott T.G. Eds. 14, 251-256.
Chang, Y. and Hudson, H.J. (1967). The fungi of wheat straw compost. 1. Ecological Studies. Trans. Brit. Mycol. Soc. 50: 649-666.
Dusterhoft, E.M., Linssen, V.A.J.M., Voragen, A.G.J. and Beldman, G. (1997). Purification, characterization and properties of two xylanase from Humicola insolens. Enzyme Microb. Technol. 20:437-445.
Flavio Henrique Moreira Souza a., Cesar Vanderlei Nascimento a., Jose´ Ce´ sar Rosa c., Douglas Chodi Masui a., Francisco Assis Leone a., Joa˜o Atı´lio Jorge b and Rosa Prazeres Melo Furriel. (2010). Purification and biochemical characterization of a mycelial glucose- and xylose-stimulated b-glucosidase from the thermophilic fungus Humicola insolens. Process biochemistry 45: 272-278.
M. Tien and T. K. Kirk. (1983). Lignin degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 22: 661-663.
Maheshwari, R., Bharadwaj, G. and Bhat, M.K. (2000). Thermophilic fungi: Their physiology and enzymes. Microbiol. Mol. Biol. Revs. 64:461–488.
Reese, E.T. (1946). Aerobic decomposition of cellulose by microorganisms at temperature above 40C, Unpublished Ph.D. Thesis. Pennsylvania state college, Evanston, Peen. 100.
Stanek, M. (1969). Effect of cellulose decomposing micro-organisms on the growth of mushrooms. Mushrooms Sci. 7: 161-171.
Sandhu, D.K. and Kalra, M.K. (1982). Production of cellulase, xylanase and pectinase by Trichoderma longibrachiatum on different substrate. Trans. Brit. Mycol. Soc. 79(3): 409-413.
Satyanaryana, T. and Johri, B.N. (1984). Thermophilic fungi of paddy straw compost: their growth, nutrition and temperature relationships. Indian J. Bot. Soc. 63: 165-170.
Vijay, B. (1996). Investigations on compost mycoflora and crop improvement in Agaricus bisporus (Lange) Sing. Ph.D. Thesis, H.P.University.
Waksman, S.A. and Cordon,T.C. (1939). Thermophilic decomposition of plant residues in composts by pure and mixed cultures of micro-organisms. Soil Sci. 47,217-224.
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