Study of genetic diversity using molecular markers in sunflower (Helianthus annuus)

RAJESH DHUTMAL; SHIV RATAN MALOO; A W MORE; VIJAY SHARMA; ANU; VIVEK K SINGH

doi:10.56093/ijas.v91i7.115129

Authors

RAJESH DHUTMAL Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan 313 001, India
SHIV RATAN MALOO Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan 313 001, India
A W MORE Sorghum Research Station, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbahni, Maharashtra
VIJAY SHARMA Banda University of Agriculture and Technology, Banda, Uttar Pradesh
ANU CCS Haryana Agricultural University, Hisar, Haryana
VIVEK K SINGH CCS Haryana Agricultural University, Hisar, Haryana

https://doi.org/10.56093/ijas.v91i7.115129

Keywords:

Genetic diversity, ISSR, RAPD, Sunflower

Abstract

Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA & T, Udaipur, Rajasthan during 2016. In the present study, RAPD primers produced 334 polymorphic band and ISSR primers produced 160 polymorphic bands. RAPD primers detected more polymorphic loci (56.88%) than the ISSR primers (52.24%). The dendrograms constructed by RAPD, ISSR primers and pooled data were associated with each other, indicating the efficacy of both marker systems in genetic diversity study of sunflower. Based on RAPD, ISSR and pooled data analysis, the restorer line EC 623023, R 16 and seed parental line CMS 234B, PET 2-7-1B were found to be genetically most diverse and fall into different groups. Therefore, their genetic origin seems to be different and could be efficiently used in sunflower breeding programs.

Downloads

Download data is not yet available.

References

Behera T K, Gaikward A B, Singh A K and Staub J E. 2008. Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). Journal of the Science of Food and Agriculture 88: 733–37. DOI: https://doi.org/10.1002/jsfa.3145

DES. 2018. Agriculture statistics at a glance 2018. Directorate of Economics and Statistics, Department of Agriculture Cooperation and Farmer’s Welfare, Govt. of India.

Dudhe M Y, Ranganatha A R G and Vishnuvardhan Reddy A. 2019. Identification of restorers and maintainers from newly developed inbreds in sunflower. Bioscience Discovery 10(1): 21–24.

Galvan M Z, Bornet B, Balatti P A and Branchard M. 2003. Inter simple sequence repeat (ISSR) marker as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica 132(3): 297–301. DOI: https://doi.org/10.1023/A:1025032622411

Garayalde A F, Poverene M, Cantamutto M and Carrera A D. 2011. Wild sunflower diversity in Argentina revealed by ISSR and SSR markers: an approach for conservation and breeding programmes. Annals of Applied Biology 158(3): 305–17. DOI: https://doi.org/10.1111/j.1744-7348.2011.00465.x

Ghany E M A. 2012. Genetic studies on sunflower using biotechnology, Ph D thesis, Cairo University, Cairo, Egypt.

Goulao L and Oliveira C M. 2011. Molecular characterization of cultivars of apple (Malus domestica Borkh.) using microsatellite (SSR and ISSR) markers. Euphytica 122: 81–89.

Iqbal M A, Sadaqat H A and Khan I A. 2008. Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis. Genetics and Molecular Research 7(4): 1408–13. DOI: https://doi.org/10.4238/vol7-4GMR494

Isaacs S M, Manivannan N and Muralidhara V. 2003. Genetic diversity analysis using RAPD markers in inbred lines of sunflower (Helianthus annuus). Helia 26(9): 59–66. DOI: https://doi.org/10.2298/HEL0339059I

Mahmoud A M and Abdel-Fatah B E. 2012. Analysis of genetic diversity among sunflower genotypes using agro-morphological traits and molecular markers. Australian Journal of Basic Applied Science 6(13): 419–32.

Maloo S R, Sharma R, Jain Devendra, Chaudhary S and Soan H. 2020. Assessment of genetic diversity in fenugreek (Trigonella foenum-graecum) genotypes using morphological and molecular markers. Indian Journal of Agricultural Sciences 90(1): 25–30. DOI: https://doi.org/10.18805/LR-4787

Mostafa G G and Alframwy A M. 2011. RAPD Analysis for detection and fingerprinting of sunflower mutants induced by sodium azide. International Journal of Plant Breeding and Genetics 5: 277–85. DOI: https://doi.org/10.3923/ijpbg.2011.277.285

Rai M. 2002. Oilseeds in India. Andhra Pradesh Agricultural Registered Research Journal : 13–15.

Reddy M P, Sarla N and Sididdiq E A. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128: 9–17.

Rohlf F J. 1998. NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.1, User Guide, Exeter software, New York.

Saghai-Maroof M A, Soliman K M, Jorgensen R A and Allard R W. 1984. Ribosomal DNAsepacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceeding of the National Acadamy of Science 81: 8014–19. DOI: https://doi.org/10.1073/pnas.81.24.8014

Seetharama A. 1981. Hybrid sunflower for higher yields. Seeds and Farms 6: 27–29.

Verma K S, Haq S U, Kkachhwaha S and Kothari S L. 2017. RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech 7(5): 288. DOI: https://doi.org/10.1007/s13205-017-0918-z

Yang J, Tang L, Ya-Li G and Wei-Bang Sun. 2012. Genetic diversity of an alien invasive plant Mexican sunflower. Weed Science 60: 552–57. DOI: https://doi.org/10.1614/WS-D-11-00175.1