Breeding, larval rearing and growth of black Amphiprion ocellaris (Cuvier, 1830) under captivity

Abstract

Broodstock development, breeding, spawning, larval rearing and growth of black Amphiprion ocellaris in captivity are
described in this study. The black ocellaris, a magnificent colour morph of A. ocellaris was bred in captivity at Vizhinjam
Research Centre of ICAR-Central Marine Fisheries Research Institute (ICAR-CMFRI). Two adults, approximately 4-6 cm
in total length (TL) were stocked in FRP tanks of 300 l capacity, under optimum water quality. The broodfishes were fed a
combination of boiled mussel meat, boiled squid and semimoist feed (protein - 40 %, lipid - 9.5 %, fibre - 2% and moisture
- 31%) twice daily at a rate of 3-5% of their body weight. After a period of 1.7 years, the fishes started showing courtship
behaviour and initiated spawning. The oocytes were of 3-3.3 mm in length and 1.1-1.9 mm in width. Approximately
200-300 eggs spawned the first time and the number of eggs gradually increased in subsequent spawnings. Spawning was
obtained at an interval of 12-14 days providing an average of two spawnings per month. The incubation period of the eggs
were 7-8 days and the fertilised eggs hatched on the 8th day, soon after sunset, generally between 19.00 and 20.00 hrs.
Different strains of cultured plankton like Isochrysis galbana, Nanochloropsis oculata, L-type rotifer Brachionus plicatilis,
calanoid copepod Acartia southwelli and Artemia were used as feed for larvae. The newly hatched larvae with a total length
of 5±0.16 mm metamorphosed to juveniles on the 40th day (18.87±0.07 mm). Black ocellaris is a highly priced clownfish
and can be used to crossbreed with the normal orange coloured ocellaris clown fish to produce different colour variants.
Keywords: Black Amphiprion ocellaris, Breeding, Broodstock development, Larval Rearing, Live feed, Variants