Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification

Shihab Ismail; N. Vineesh; C. Mohitha; P. Vijayagopal; A. Gopalakrishnan

doi:10.21077/

Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification

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Authors

Shihab Ismail Central Marine Fisheries Research Institute
N. Vineesh Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar
C. Mohitha Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar
P. Vijayagopal Central Marine Fisheries Research Institute Marine Biotechnology Division
A. Gopalakrishnan Central Marine Fisheries Research Institute

https://doi.org/10.21077/

Abstract

The four fingered threadfin Eleutheronema tetradactylum (Shaw, 1804) is a prioritised species for mariculture in India. Their demand in the domestic markets is rapidly growing. Genetic stock structure analysis of fish populations is an important aspect from fisheries management perspective. The present study was conducted to develop microsatellite primers through cross-priming to elucidate the genetic structure of E. tetradactylum. A total of 13 polymorphic microsatellite markers were developed from the resource species, Pacific salmon Polydactylus sexfilis. The observed mean and the effective number of alleles were found to be 11.962 and 6.927 respectively. The mean of observed heterozygosity (Ho) and expected heterozygosity (He) values obtained were 0.784 and 0.798 respectively. These new microsatellite markers can be used as effective tools for studying genetic disparity as well as for elucidating evolutionary relationships among E. tetradactylum populations.

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Author Biographies

Shihab Ismail, Central Marine Fisheries Research Institute

Marine Biotechnology Division
Research Scholar
N. Vineesh, Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar

Central Marine Fisheries Research Institute
Marine Biotechnology Division
Research Scholar
C. Mohitha, Central Marine Fisheries Research Institute Marine Biotechnology Division Research Scholar

Central Marine Fisheries Research Institute
Marine Biotechnology Division
Research Scholar
P. Vijayagopal, Central Marine Fisheries Research Institute Marine Biotechnology Division

Central Marine Fisheries Research Institute
Marine Biotechnology Division
Principal Scientist
Â
A. Gopalakrishnan, Central Marine Fisheries Research Institute

Central Marine Fisheries Research Institute
Director

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Submitted

2018-07-23

Published

2018-09-30

Issue

Vol. 65 No. 3 (2018)

Section

Articles

License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

The copyright of the articles published in Indian Journal of Fisheries vests with the Indian Council of Agricultural Research, who has the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in these journals. The Council has no objection in using the material, provided the information is being utilized for academic purpose but not for commercial use. Due credit line should be given to the ICAR where information will be utilized.

How to Cite

Ismail, S., Vineesh, N., Mohitha, C., Vijayagopal, P., & Gopalakrishnan, A. (2018). Development and validation of microsatellite markers in a protandrous fish species Eleutheronema tetradactylum (Shaw, 1804) through cross-species amplification. Indian Journal of Fisheries, 65(3). https://doi.org/10.21077/